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SKU:PAB897Ra01

Polyclonal Antibody to Insulin Degrading Enzyme (IDE)

Polyclonal Antibody to Insulin Degrading Enzyme (IDE)

Regular price $362.00 USD
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UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAB897Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml




SPECIFITY

The antibody is a rabbit polyclonal antibody raised against IDE. It has been selected for its ability to recognize IDE in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
J Neurochem Reduction PubMed: 26442993
African Journal of Pharmacy and Pharmacology Peptide hydroxamate derivatives as regulators for insulin receptor signaling and its degradation by zinc metalloprotease in diabetic rats article-full-text-pdf
Biomedicine & Pharmacotherapy Vitamin D3 intake as regulator of insulin degrading enzyme and insulin receptor phosphorylationin diabetic rats. pubmed:27930980
Biological Trace Element Research Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats 10.1007/s12011-017-1167-z
Neuroscience The Protective Effects of IGF-I against β-Amyloid-related Downregulation of Hippocampal Somatostatinergic System Involve Activation of Akt and Protein Kinase A Pubmed:29406271
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB897Ra01 Recombinant Insulin Degrading Enzyme (IDE) Positive Control; Immunogen; SDS-PAGE; WB.
PAB897Ra01 Polyclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
MAB897Ra21 Monoclonal Antibody to Insulin Degrading Enzyme (IDE) WB; IHC; ICC; IP.
SEB897Ra ELISA Kit for Insulin Degrading Enzyme (IDE) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB897Ra Magnetic Luminex Assay Kit for Insulin Degrading Enzyme (IDE) ,etc. Magnetic Luminex Assay for Antigen Detection


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