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SKU:PAB929Ra01

Polyclonal Antibody to Choline Acetyltransferase (ChAT)

Polyclonal Antibody to Choline Acetyltransferase (ChAT)

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UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Product No.

PAB929Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB929Ra01-Recombinant Choline Acetyltransferase (ChAT)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies

UOM

20µl 100µl 200µl 1ml 10ml



SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ChAT. It has been selected for its ability to recognize ChAT in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Neurochemical Research Trans-resveratrol Inhibits Tau Phosphorylation in the Brains of Control and Cadmium Chloride-Treated Rats by Activating PP2A and PI3K/Akt Induced-Inhibition of … Pubmed: 30478674
neurotoxicity research Melatonin Improves Memory Deficits in Rats with Cerebral Hypoperfusion, Possibly, Through Decreasing the Expression of Small-Conductance Ca2+-Activated K+ … Pubmed: 31187398
BMC Anesthesiology Cerebrospinal fluid cholinergic biomarkers are associated with postoperative delirium in elderly patients undergoing Total hip/knee replacement: a … Pubmed: 32988385
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB929Ra01 Recombinant Choline Acetyltransferase (ChAT) Positive Control; Immunogen; SDS-PAGE; WB.
PAB929Ra01 Polyclonal Antibody to Choline Acetyltransferase (ChAT) WB; IHC; ICC; IP.
MAB929Ra21 Monoclonal Antibody to Choline Acetyltransferase (ChAT) WB; IHC; ICC; IP.
SEB929Ra ELISA Kit for Choline Acetyltransferase (ChAT) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB929Ra CLIA Kit for Choline Acetyltransferase (ChAT) Chemiluminescent immunoassay for Antigen Detection.
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