Cloud-clone
SKU:PAC027Hu01
Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)
Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)
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Immunohistochemistry: 5-20µg/mL;1:20-80
Immunocytochemistry: 5-20µg/mL;1:20-80
Optimal working dilutions must be determined by end user.
Product No.
PAC027Hu01
Organism Species
Homo sapiens (Human)
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPC027Hu01-Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
n/a
Applications
WB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MGMT. It has been selected for its ability to recognize MGMT in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Biotechnology and Bioengineering | Targeted nitric oxide delivery preferentially induces glioma cell chemosensitivity via altered p53 and O(6) -Methylguanine-DNA Methyltransferase activity PubMed: 23125026 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC027Hu01 | Recombinant O-6-Methylguanine DNA Methyltransferase (MGMT) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC027Hu01 | Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) | WB,IHC |
| MAC027Hu22 | Monoclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT) | WB; IHC; ICC; IP. |
| SEC027Hu | ELISA Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC027Hu | Magnetic Luminex Assay Kit for O-6-Methylguanine DNA Methyltransferase (MGMT) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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