Cloud-clone
SKU:MAC123Hu22
Monoclonal Antibody to SMAD family member 3 (SMAD3)
Monoclonal Antibody to SMAD family member 3 (SMAD3)
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;Immunohistochemistry: 5-20µg/mL;1:50-200
;Immunocytochemistry: 5-20µg/mL;1:50-200
;Optimal working dilutions must be determined by end user.
Product No.
MAC123Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
C4
Ig Isotype
IgG2b Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPC123Hu01-Recombinant SMAD family member 3 (SMAD3)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Sus scrofa; Porcine (Pig)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against SMAD3. It has been selected for its ability to recognize SMAD3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| У¿¯ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC123Hu01 | Recombinant SMAD family member 3 (SMAD3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Polyclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu21 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu22 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu23 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
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