Cloud-clone
SKU:MAC143Hu22
Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)
Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)
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Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAC143Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
D2
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPC143Hu01-Recombinant Collagen Type IV Alpha 3 (COL4a3)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.65mg/ml
Organism Species More
Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against COL4a3. It has been selected for its ability to recognize COL4a3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Experimental and Molecular Pathology | Epitope recognized by anti-glomerular basement membrane (GBM) antibody in a patient with repeated relapse of anti-GBM disease Pubmed: 30817909 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC143Hu02 | Recombinant Collagen Type IV Alpha 3 (COL4a3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC143Hu01 | Recombinant Collagen Type IV Alpha 3 (COL4a3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC143Hu01 | Polyclonal Antibody to Collagen Type IV Alpha 3 (COL4a3) | WB; IHC; ICC; IP. |
| MAC143Hu22 | Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3) | WB; IHC; ICC; IP. |
| MAC143Hu21 | Monoclonal Antibody to Collagen Type IV Alpha 3 (COL4a3) | WB; IHC; ICC; IP. |
| SEC143Hu | ELISA Kit for Collagen Type IV Alpha 3 (COL4a3) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC143Hu | Magnetic Luminex Assay Kit for Collagen Type IV Alpha 3 (COL4a3) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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