Cloud-clone
SKU:PAC242Mu01
Polyclonal Antibody to Nucleolin (NCL)
Polyclonal Antibody to Nucleolin (NCL)
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;Immunohistochemistry: 5-20µg/mL;1:25-110
;Immunocytochemistry: 5-20µg/mL;1:25-110
;Optimal working dilutions must be determined by end user.
Product No.
PAC242Mu01
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPC242Mu01-Recombinant Nucleolin (NCL)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Homo sapiens (Human), Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NCL. It has been selected for its ability to recognize NCL in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| European Journal of Pharmaceutics and Biopharmaceutics | RNA G-quadruplex as supramolecular carrier for cancer-selective delivery |
| Mol Pharm | Drug Loading and Functional Efficacy of Cow, Buffalo, and Goat Milk-Derived Exosomes: A Comparative Study Pubmed:35195427 |
| Frontiers in Physiology | Fine Tuning Inclusion in 2 of′-O-Methyl Phosphorothioate Oligonucleotides Contributes to Specific Cell Targeting for Splice-Switching Modulation Pubmed:34721051 |
| Catalog No. | Related products for research use of Mus musculus (Mouse) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC242Mu01 | Recombinant Nucleolin (NCL) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC242Mu01 | Polyclonal Antibody to Nucleolin (NCL) | WB; IHC; ICC; IP. |
| LAC242Mu71 | Biotin-Linked Polyclonal Antibody to Nucleolin (NCL) | WB; IHC; ICC. |
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