Cloud-clone
SKU:MAC243Hu22
Monoclonal Antibody to Topoisomerase I (TOP1)
Monoclonal Antibody to Topoisomerase I (TOP1)
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Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
Product No.
MAC243Hu22
Organism Species
Homo sapiens (Human)
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
n/a
Ig Isotype
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against TOP1. It has been selected for its ability to recognize TOP1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Electronic Journal of Biotechnology | Comparing the expression of human DNA topoisomerase I in KM71H and X33 strains of Pichia pastoris science:S0717345816000233 |
| Journal of Experimental & Clinical Cancer Research | Inhibitory effects of lapachol on rat C6 glioma in vitro and in vivo by targeting DNA topoisomerase I and topoisomerase II pubmed:27852319 |
| Tikrit Journal of Pure Science | Partial purification of topoisomerase IB from serum of diabetic patients and study it's kinetic properties and molecular weight |
| EurAsian Journal of BioSciences | Study of topoisomerase I (Topo I) level and some biochemical parameters in diabetic patients |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC243Hu01 | Recombinant Topoisomerase I (TOP1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC243Hu01 | Polyclonal Antibody to Topoisomerase I (TOP1) | WB; IHC; ICC; IP. |
| MAC243Hu22 | Monoclonal Antibody to Topoisomerase I (TOP1) | WB; IHC; ICC; IP. |
| SEC243Hu | ELISA Kit for Topoisomerase I (TOP1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC243Hu | Magnetic Luminex Assay Kit for Topoisomerase I (TOP1) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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