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SKU:PAC262Hu01

Polyclonal Antibody to Tight Junction Protein 1 (TJP1)

Polyclonal Antibody to Tight Junction Protein 1 (TJP1)

Regular price $350.00 USD
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UOM
Concentration
Organism species
Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

PAC262Hu01

Organism Species

Homo sapiens (Human) 

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPC262Hu01-Recombinant Tight Junction Protein 1 (TJP1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a rabbit polyclonal antibody raised against TJP1. It has been selected for its ability to recognize TJP1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Current Nutrition & Food Science Barrier Strengthening and Anti-inflammatory Effect of Cucurbit Fruits on Intestinal Epithelial Cells In-vitro 10.2174:1573401313666170427123153
Journal of Translational Medicine Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation Pubmed: 33036625
Neural Regeneration Research Inhibition of nitric oxide synthase aggravates brain injury in diabetic rats with traumatic brain injury 33433486
PLoS One A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … 34492054
J Agric Food Chem Pyocyanin Modulates Gastrointestinal Transformation and Microbiota Pubmed:35171599
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