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SKU:MAC302Hu22

Monoclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1)

Monoclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1)

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UOM
Concentration
Organism species
Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

MAC302Hu22

Organism Species

Homo sapiens (Human) 

Source

Monoclonal antibody preparation

Host

Mouse

Potency (Clone Number)

n/a

Ig Isotype

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

1mg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml


SPECIFITY

The antibody is a mouse monoclonal antibody raised against ASAH1. It has been selected for its ability to recognize ASAH1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine Citations
Cytotechnology The investigation of ceranib-2 on apoptosis and drug interaction with carboplatin in human non small cell lung cancer cells in vitro pubmed:29230631
Cells Targeting Acid Ceramidase to Improve the Radiosensitivity of Rectal Cancer 33334013
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC302Hu01 Recombinant N-Acylsphingosine Amidohydrolase 1 (ASAH1) Positive Control; Immunogen; SDS-PAGE; WB.
PAC302Hu01 Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1) WB; IHC; ICC; IP.
MAC302Hu22 Monoclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1) WB; IHC; ICC; IP.
SEC302Hu ELISA Kit for N-Acylsphingosine Amidohydrolase 1 (ASAH1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC302Hu Magnetic Luminex Assay Kit for N-Acylsphingosine Amidohydrolase 1 (ASAH1) ,etc. Magnetic Luminex Assay for Antigen Detection.


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