Cloud-clone
SKU:PAC332Hu01
Polyclonal Antibody to Nuclear Mitotic Apparatus Protein 1 (NUMA1)
Polyclonal Antibody to Nuclear Mitotic Apparatus Protein 1 (NUMA1)
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Western blotting: 0.5-2µg/mL;1:200-800
;Immunohistochemistry: 5-20µg/mL;1:20-80
;Immunocytochemistry: 5-20µg/mL;1:20-80
;Optimal working dilutions must be determined by end user.
Product No.
PAC332Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPC332Hu01-Recombinant Nuclear Mitotic Apparatus Protein 1 (NUMA1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
Mus musculus (Mouse), Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NUMA1. It has been selected for its ability to recognize NUMA1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Oncotarget | Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer inurine samples. pubmed:27203388 |
| Journal of Micromechanics and Microengineering | A simple fabricated microfluidic chip for urine sample-based bladder cancer detection |
| Journal of Electroanalytical Chemistry | A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample |
| Electrophoresis | Bladder cancer hunting: a microfluidic paper‐based analytical device Pubmed: 32530061 |
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