Cloud-clone
SKU:MAC332Hu22
Monoclonal Antibody to Nuclear Mitotic Apparatus Protein 1 (NUMA1)
Monoclonal Antibody to Nuclear Mitotic Apparatus Protein 1 (NUMA1)
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Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
Product No.
MAC332Hu22
Organism Species
Homo sapiens (Human).
Source
Monoclonal antibody preparation
Host
Mouse
Potency (Clone Number)
D9
Ig Isotype
IgG1 Kappa
Purification
Protein A + Protein G affinity chromatography
Label
None
Immunogen
RPC332Hu01-Recombinant Nuclear Mitotic Apparatus Protein 1 (NUMA1)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
1mg/ml
Organism Species More
Sus scrofa; Porcine (Pig)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a mouse monoclonal antibody raised against NUMA1. It has been selected for its ability to recognize NUMA1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Oncotarget | Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer inurine samples. pubmed:27203388 |
| Journal of Micromechanics and Microengineering | A simple fabricated microfluidic chip for urine sample-based bladder cancer detection |
| Journal of Electroanalytical Chemistry | A highly sensitive label-free electrochemical immunosensor based on AuNPs-PtNPs-MOFs for nuclear matrix protein 22 analysis in urine sample |
| Electrophoresis | Bladder cancer hunting: a microfluidic paper‐based analytical device Pubmed: 32530061 |
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