Cloud-clone
SKU:PAC559Ra01
Polyclonal Antibody to Kisspeptin 1 (KISS1)
Polyclonal Antibody to Kisspeptin 1 (KISS1)
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Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Product No.
PAC559Ra01
Organism Species
Rattus norvegicus (Rat).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
EPC559Ra61-Eukaryotic Kisspeptin 1 (KISS1)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against KISS1. It has been selected for its ability to recognize KISS1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Mol Endocrinol. |
LIM-homeodomain transcription factor Isl-1 mediates kisspeptin's effect on insulin secretion in mice. Pubmed:24956377 |
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临床儿科杂志 |
双酚A 与女童性早熟的关系 Fileup: Pdf |
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J Pediatr Endocrinol Metab |
The relation of urinary bisphenol A with kisspeptin in girls diagnosed with central precocious puberty and premature thelarche PubMed: 26565542 |
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Archives of Physiology and Biochemistry |
Circulating levels of leptin, nesfatin-1 and kisspeptin in postmenopausal obese women Pubmed:27011110 |
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International Journal of Endocrinology |
The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages pubmed:27990162 |
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Journal of Clinical Research in Pediatric Endocrinology |
Basal Serum Neurokinin B Levels in Differentiating Idiopathic Central Precocious Puberty from Premature Thelarche. pubmed:28008860 |
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BioMed Research International |
Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China |
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OBESITY SURGERY |
Lack of Improvement of Sperm Characteristics in Obese Males After Obesity Surgery Despite the Beneficial Changes Observed in Reproductive Hormones Pubmed: 30806913 |
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International Journal of Endocrinology |
Serum Kisspeptin and Its Relation to Metabolic Parameters and Glucose Metabolism in Prepubertal and Pubertal Obese Children Pubmed: 33224197 |
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journal of reproduction and fertility |
KISS1R Signaling Modulates Gonadotropin Sensitivity in Mouse Leydig Cell Pubmed: 33112765 |
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Beyond GnRH, LH and FSH: the role of kisspeptin on hypothalalmic-3 pituitary gonadal (HPG) axis pathology and diagnostic consideration 4 |
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Medeni Med J |
Kisspeptin and Hematologic Parameters as Predictive Biomarkers for First-Trimester Abortions 34239761 |
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Arch Gynecol Obstet |
Comparison of the Kisspeptin levels in early onset preeclampsia and late-onset preeclampsia Pubmed:35039882 |
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J Pediatr Endocrinol Metab |
Serum kisspeptin, leptin, neuropeptide Y, and neurokinin B levels in adolescents with polycystic ovary syndrome Pubmed:35170267 |
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Genes |
Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis Pubmed:35205340 |
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Catalog No. |
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