Cloud-clone
SKU:PAC992Hu01
Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)
Polyclonal Antibody to Glutathione Peroxidase 3, Plasma (GPX3)
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Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.
Product No.
PAC992Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPC992Hu01-Recombinant Glutathione Peroxidase 3, Plasma (GPX3)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
n/a
Applications
WB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against GPX3. It has been selected for its ability to recognize GPX3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Reproduction |
Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis Pubmed:25376627 |
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international journal of cardiology |
Endothelial function impairment in STEMI patients with out-of-hospital cardiac arrest under therapeutic hypothermia treatment. pubmed:28087172 |
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Current Alzheimer Research |
Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients. pubmed:27748187 |
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Frontiers in Microbiology |
Burn Serum Increases Staphylococcus aureus Biofilm Formation via Oxidative Stress. pubmed:28702016 |
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Clinical and Translational Oncology |
Hydrogen peroxide-induced mitophagy contributes to laryngeal cancer cells survival via the upregulation of FUNDC1 Pubmed: 30284230 |
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Toxicological Sciences |
Kidney toxicity and response of selenium containing protein-glutathione peroxidase (Gpx3) to CdTe QDs on different levels Pubmed: 30535317 |
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Available at SSRN 3234834 |
Human Plasma Protein (s) Based Conceptual Diagnostic Tool for Altitude-Acclimation Assessment |
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The Journal of Nutritional Biochemistry |
Dietary selenium deficiency or selenomethionine excess drastically alters organ selenium contents without altering the expression of most selenoproteins in mice |
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Ecotoxicology and Environmental Safety |
Molecular mechanisms of lead-induced changes of selenium status in mice livers through interacting with selenoprotein P |
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY |
In vivo and in vitro studies on inactivation of selenium containing protein-glutathione peroxidase 3 in mice nephrocytes caused by lead Pubmed: 32678766 |
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Nat Immunol |
Selenium–GPX4 axis protects follicular helper T cells from ferroptosis 34413521 |
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Eberhard Karls University |
Experimental nephrotic syndrome in mice with an inducible deletion of podocin and the role of plasminogen in sodium retention |
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Catalog No. |
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Applications (RESEARCH USE ONLY!) |
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