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SKU:PAE038Hu01

Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL)

Polyclonal Antibody to Neurofilament, Light Polypeptide (NEFL)

Regular price $350.00 USD
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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAE038Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPE038Hu01-Recombinant Neurofilament, Light Polypeptide (NEFL)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.69mg/ml

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against NEFL. It has been selected for its ability to recognize NEFL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Acta Neurologica Scandinavica

Effect of carotid endarterectomy on brain damage markers Pubmed:27126899

Acta Neurologica Scandinavica

Effect of carotid endarterectomy on brain damage markers. pubmed:27126899

journal of neuroimmunology

Cytokines and biological markers in autoimmune GFAP astrocytopathy: The potential role for pathogenesis and therapeutic implications Pubmed: 31254929

Cerebellum

Neurofilament Light Chain Is a Biomarker of Neurodegeneration in Ataxia Telangiectasia 33893614

Medicine (Baltimore)

Phase 1b dose-escalation, safety, and pharmacokinetic study of IC14, a monoclonal antibody against CD14, for the treatment of amyotrophic lateral sclerosis 34678870

J Clin Med

Fatty Acid-Binding Protein 7 (FABP-7), Glutamic Acid and Neurofilament Light Chain (NFL) as Potential Markers of Neurodegenerative Disorders in Psoriatic Patients … Pubmed:35566558


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