Cloud-clone
SKU:PAG515Hu01
Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)
Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAG515Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPG515Hu01-Recombinant Chymase 1, Mast Cell (CMA1)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CMA1. It has been selected for its ability to recognize CMA1 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Pathobiology |
Anti-Angiotensin and Hypoglycemic Treatments Suppress Liver Metastasis of Colon Cancer Cells Karger: 000330169 |
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Clinical and Experimental Metastasis |
Diabetes-associated angiotensin activation enhances liver metastasis of colon cancer PubMed: 22552372 |
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Mediators of Inflammation |
Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis Pubmed:25400334 |
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Neurogastroenterology & Motility |
Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery Pubmed:25677271 |
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IJC Metab Endocr |
The autocrine role of tryptase in pressure overload-induced mast cell activation, chymase release and cardiac fibrosis PubMed: 26722642 |
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Journal of Neuroinflammation |
Oral sensitization to whey proteins induces age-and sex-dependent behavioral abnormality and neuroinflammatory responses in a mouse model of food … Pubmed:29685134 |
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Revista do Instituto de Medicina Tropical de São Paulo |
Vasoactive intestinal peptide degradation might influence Interleukin-17 expression in cardiac chagasic patients Pubmed: 30365640 |
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Science Advances |
Platelets trigger perivascular mast cell degranulation to cause inflammatory responses and tissue injury Pubmed: 32206714 |
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Immunity Inflammation and Disease |
Disruption in the balance between apolipoprotein A‐I and mast cell chymase in chronic hypersensitivity pneumonitis Pubmed: 33016012 |
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Microbiol Spectr |
Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome Pubmed:35612327 |
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Catalog No. |
Related products for research use of Homo sapiens (Human) Organism species |
Applications (RESEARCH USE ONLY!) |
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RPG515Hu01 |
Positive Control; Immunogen; SDS-PAGE; WB. |
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PAG515Hu01 |
WB; IHC; ICC; IP. |
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PAG515Hu02 |
WB; IHC; ICC; IP. |
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MAG515Hu21 |
WB; IHC; ICC; IP. |
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SEG515Hu |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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LMG515Hu |
Magnetic Luminex Assay Kit for Chymase 1, Mast Cell (CMA1) ,etc. |
Magnetic Luminex Assay for Antigen Detection. |
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