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SKU:PAK202Hu01
Polyclonal Antibody to A Disintegrin And Metalloprotease 28 (ADAM28)
Polyclonal Antibody to A Disintegrin And Metalloprotease 28 (ADAM28)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAK202Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPK202Hu01-Recombinant A Disintegrin And Metalloprotease 28 (ADAM28)
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ADAM28. It has been selected for its ability to recognize ADAM28 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Rapid Communications in Mass Spectrometry |
Urinary protein profiling by liquid chromatography/tandem mass spectrometry: ADAM28 is overexpressed in bladder transitional cell carcinoma Pubmed: 21913264 |
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Medical Oncology |
Baseline and decline of serum ADAM28 during chemotherapy of advanced non-small cell lung cancer: a probable predictive and prognostic factor SpringerLink: kg628446760r60rl |
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Immunology & Cell Biology |
ADAM28 is elevated in humans with the metabolic syndrome and is a novel sheddase of human tumour necrosis factor-α. PubMed: 23010875 |
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Leuk Res |
ADAM28 overexpression regulated via the PI3K/Akt pathway is associated with relapse in PubMed: 26340916 |
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Disease Markers |
Adamalysines as Biomarkers and a Potential Target of Therapy in Colorectal Cancer Patients: Preliminary Results Pubmed: 31565100 |
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Catalog No. |
Related products for research use of Homo sapiens (Human) Organism species |
Applications (RESEARCH USE ONLY!) |
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RPK202Hu01 |
Positive Control; Immunogen; SDS-PAGE; WB. |
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PAK202Hu01 |
Polyclonal Antibody to A Disintegrin And Metalloprotease 28 (ADAM28) |
WB; IHC; ICC; IP. |
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MAK202Hu21 |
Monoclonal Antibody to A Disintegrin And Metalloprotease 28 (ADAM28) |
WB; IHC; ICC; IP. |
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SEK202Hu |
Enzyme-linked immunosorbent assay for Antigen Detection. |
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LMK202Hu |
Magnetic Luminex Assay Kit for A Disintegrin And Metalloprotease 28 (ADAM28) ,etc. |
Magnetic Luminex Assay for Antigen Detection. |
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