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SKU:PAB499Hu01

Polyclonal Antibody to Tumor Necrosis Factor Receptor 1 (TNFR1)

Polyclonal Antibody to Tumor Necrosis Factor Receptor 1 (TNFR1)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

PAB499Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB499Hu01-Recombinant Tumor Necrosis Factor Receptor 1 (TNFR1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against TNFR1. It has been selected for its ability to recognize TNFR1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

PLoS ONE

A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches Plosone: Source

Mediators of Inflammation

High Glucose Level Disturbs the Resveratrol-Evoked Curtailment of CX3CL1/CX3CR1 Signaling in Human Placental Circulation pubmed:28655972

Annals of Neurology 

High complement levels in astrocyte‐derived exosomes of Alzheimer disease Pubmed:29406582

International Journal of Immunopathology and Pharmacology

VEGF-R2 and TNF-R1 expression and cytokine production by samples of mammary adenocarcinomas and correlations with histopathological parameters of these … Pubmed:29985074

Cell Death & Disease

Inhibition of transmembrane TNF-α shedding by a specific antibody protects against septic shock Pubmed: 31383857

Journal of Cardiovascular Translational Research

XPO1 Gene Therapy Attenuates Cardiac Dysfunction in Rats with Chronic Induced Myocardial Infarction Pubmed: 31768947

Frontiers in Pharmacology

M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice Pubmed: 33041798


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