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SKU:PAA585Ga01

Polyclonal Antibody to Cyclin D1 (CCND1)

Polyclonal Antibody to Cyclin D1 (CCND1)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Product No.

PAA585Ga01

Organism Species

Chicken (Gallus).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA585Ga01-Recombinant Cyclin D1 (CCND1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CCND1. It has been selected for its ability to recognize CCND1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Biochemical Pharmacology

Antiproliferative and proapoptotic activity of CLM3, a novel multiple tyrosine kinase inhibitor, alone and in combination with SN-38 on endothelial and cancer cells ScienceDirect: S0006295211002097

Leukemia & Lymphoma

The nanoparticulate Quillaja saponin KGI exerts anti-proliferative effects by down-regulation of cell cycle molecules in U937 and HL-62 human leukemia cells Pubmed: 24138332

The Journal of Clinical Endocrinology and Metabolism

Antiproliferative and proapoptotic activity of sunitinib on endothelial and anaplastic thyroid cancer cells via inhibition of Akt and ERK1/2 phosphorylation and by down-regulation of cyclin-D1. Pubmed: 23969186

Nutrition and Cancer

Common Food Additive Carrageenan Stimulates Wnt/ β-Catenin Signaling in Colonic Epithelium by Inhibition of Nucleoredoxin Reduction Pubmed:24328990

The International Journal of Biochemistry & Cell Biology

MicroRNA-149 is epigenetically silenced tumor-suppressive microRNA, involved in cell proliferation and downregulation of AKT1 and cyclin D1 in human … pubmed:27783537

Scientific Reports

Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA. pubmed:28533512

Investigational new drugs

Development of a dietary formulation of the SHetA2 chemoprevention drug for mice. pubmed:29273857

Biochemical Pharmacology

Metronomic vinorelbine is directly active on Non Small Cell Lung Cancer cells and sensitizes the EGFRL858R/T790M cells to reversible EGFR tyrosine kinase … Pubmed:29660315

Apoptosis

Licarin A induces cell death by activation of autophagy and apoptosis in non-small cell lung cancer cells Pubmed:29468481

Oncology Reports

Vandetanib has antineoplastic activity in anaplastic thyroid cancer, in vitro and in vivo Pubmed:29517106

Oncology Reports

Lenvatinib exhibits antineoplastic activity in anaplastic thyroid cancer in vitro and in vivo Pubmed:29517103

Scientific Reports

Is there an association between enhanced choline and β-catenin pathway in breast cancer? A pilot study by MR Spectroscopy and ELISA Pubmed: 28533512

Middle East Journal of Rehabilitation and Health

Antiapoptotic Effects of Continuous Training and Selenium Consumption on the Liver Tissue of Cadmium-Exposed Rats Pubmed: 10.5812/mejrh.91278

European journal of medicinal chemistry

Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity Pubmed: 31216504


Catalog No.

Related products for research use of Chicken (Gallus) Organism species

Applications (RESEARCH USE ONLY!)

RPA585Ga01

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Positive Control; Immunogen; SDS-PAGE; WB.

PAA585Ga01

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WB; IHC; ICC; IP.

LAA585Ga71

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WB; IHC; ICC.

LAA585Ga81

FITC-Linked Polyclonal Antibody to Cyclin D1 (CCND1)

WB; IHC; ICC; IF.

MAA585Ga21

Monoclonal Antibody to Cyclin D1 (CCND1)

WB; IHC; ICC; IP.

 

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