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SKU:PAA777Ra01

Polyclonal Antibody to Inducible T-Cell Co Stimulator (ICOS)

Polyclonal Antibody to Inducible T-Cell Co Stimulator (ICOS)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAA777Ra01

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ICOS. It has been selected for its ability to recognize ICOS in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Archives of Dermatological Research

Increased production of soluble inducible costimulator in patients with diffuse cutaneous systemic sclerosis Pubmed: 23053221

DNA Cell Biol.

Effect of soluble inducible costimulator level and its polymorphisms on age-related macular degeneration. Pubmed: 24083358

Rheumatology

Augmented ICOS expression in patients with early diffuse cutaneous systemic sclerosis Pubmed: 23024058

Br J Haematol

Inducible T‐cell co‐stimulator (ICOS) and ICOS ligand are novel players in the multiple‐myeloma microenvironment Pubmed:34954822

Diagnostics (Basel)

Increased Levels of ICOS and ICOSL Are Associated to Pulmonary Arterial Hypertension in Patients Affected by Connective Tissue Diseases Pubmed:35328257


Catalog No.

Related products for research use of Rattus norvegicus (Rat) Organism species

Applications (RESEARCH USE ONLY!)

RPA777Ra01

Recombinant Inducible T-Cell Co Stimulator (ICOS)

Positive Control; Immunogen; SDS-PAGE; WB.

PAA777Ra01

Polyclonal Antibody to Inducible T-Cell Co Stimulator (ICOS)

WB; IHC; ICC; IP.

LAA777Ra71

Biotin-Linked Polyclonal Antibody to Inducible T-Cell Co Stimulator (ICOS)

WB; IHC; ICC.

 

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