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SKU:PAA830Mu01

Polyclonal Antibody to Follicle Stimulating Hormone (FSH)

Polyclonal Antibody to Follicle Stimulating Hormone (FSH)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAA830Mu01

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA830Mu01-Recombinant Follicle Stimulating Hormone (FSH)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against FSH. It has been selected for its ability to recognize FSH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

International Journal of Clinical and Experimental Medicine

Studies of the pharmacology of 17α-ethynyl-androst-5-ene-3β,7β,17β-triol, a synthetic anti-inflammatory androstene Ijcem: IJCEM1102004

Science Alert

Reproductive Toxicity of Tobacco Shisha Smoking on Semen Parameters and Hormones Levels among Adult Egyptian Men SciAlert: source

EXCLI Journal

Protective role of Hibiscus sabdariffa calyx extract against streptozotocin induced sperm damage in diabetic rats Excli: Source

International Journal of Molecular and Cellular Medicine

Central effects of camphor on GnRH and sexual hormones in male rat Pubmed: 24551777

Urologia internationalis

Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats Pubmed: 24051982

Electromagnetic Biology and Medicine

The effects of long-term exposure to a 2450 MHz electromagnetic field on growth and pubertal development in female Wistar rats Informahealthcare: Source

Academic Sciences

Piper longum hexane fraction induces infertility by modulation of inflammatory mediators and gonadotropin insufficiency in female rats Ijppsjournal: Source

Mol Cell Endocrinol.

Quercetin supplementation restores testicular function and augments germ cell survival in the estrogenized rats Pubmed:24316378

Endocrinology

FoxO1 Is a Negative Regulator of FSHβ Gene Expression in Basal and GnRH-Stimulated Conditions in Female Pubmed:24437485

Andrologia

Protective effects of Eugenia jambolana extract versus N-acetyl cysteine against cisplatin-induced damage in rat testis Pubmed:24576220

Environmental Toxicology and Pharmacology

Endocrine disrupting effects of dichlorodiphenyltrichloroethane analogues on gonadotropin hormones in pituitary gonadotrope cells Pubmed:24814263

Environmental Research

Perfluorooctane sulfonate effects on the reproductive axis in adult male rats Pubmed:25171141

Genet Mol Biol.

Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility Pubmed:25505846

Turkish Journal of Urology

Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy Turkishjournalofurology:Source

oncology research

Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940

Andrologia

Protective effects of Eugenia jambolana extract versus N‐acetyl cysteine against cisplatin‐induced damage in rat testis PubMed: 24576220

European Journal of Pharmacology

The effect of R-(-)-deprenyl administration on reproductive parameters of rat males PubMed: 25725114

Andrologia

Dose‐and time‐dependent effects of Garcinia kola seed extract on sexual behaviour and reproductive parameters in male Wistar rats PubMed: 26123866

Reprod Toxicol

Simvastatin decreases steroid production in the H295R cell line and decreases steroids and FSH in female rats PubMed: 26476359

The Journal of The Society for Reproduction and Fertility

Sex-specific prenatal stress effects on the rat reproductive axis and adrenal gland structure Pubmed:27026714

Biology of Reproduction

Maternal High-Fat Diet Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring Pubmed:26962114

Andrologia

Dose- and time-dependent effects of Garcinia kola seed extract on sexual behaviour and reproductiveparameters in male Wistar rats. Pubmed:26123866

Scientific Reports

Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus pubmed:27461373

33

Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome. pubmed:28336389

environmental pollution

Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061

Andrologia

Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats. pubmed:28000943

Biology of Reproduction

Maternal High-Fat Diet-Induced Loss of Fetal Oocytes Is Associated with Compromised Follicle Growth in Adult Rat Offspring. pubmed:26962114

J Endocrinol.

Impact of uteroplacental insufficiency on postnatal rat male gonad. pubmed:27885054

Int J Pharm.

New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency. pubmed:28232269

Environmental Science & Technology

Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis pubmed:28731686

Journal of Medicinal Chemistry

Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity pubmed:28958147

Society of Toxicology

Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LbT2 Mouse Pituitary Cells pubmed:29149324

Reproductive Toxicology

Vitamin D deficiency impairs testicular development and spermatogenesis in mice pubmed:28655646

The Journal of Biological Chemistry.

Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis. pubmed:28578315

The Journal of Toxicological Sciences

High sensitivity of testicular function to titanium nanoparticles pubmed:28496042

Journal of Orthopaedic Research

The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy‐induced osteoporotic mice pubmed:29058779

DARU Journal of Pharmaceutical Sciences

Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway pubmed:29020999

Reproduction.

Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593

Annals of Rheumatic Diseases 

FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract

Stem Cell Research & Therapy

The role of mesenchymal stem cells in chemotherapy-induced gonadotoxicity Pubmed:30021657

Environmental Pollution

Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625

Frontiers in Molecular Neuroscience

Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice Pubmed:29403355

Biology of Reproduction

Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580

Journal of Ovarian Research

DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway Pubmed:29321035

Medical Science Monitor

Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) … Pubmed:29627845

Human & Experimental Toxicology

Palladium nanoparticle effects on endocrine reproductive system of female rats Pubmed:29405765


A Pex13 knockout in germ cells induces a spermatogenic arrest volltexte:2018/8921/

Biomedicine & Pharmacotherapy

Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons Doi: 10.1016/j.biopha.2018.08.135

Stem Cells Translational Medicine

Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats Pubmed: 30569668

Journal of Endocrinology

Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome Pubmed: 29535146

Experimental and Therapeutic Medicine

Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary

Biomedicine & Pharmacotherapy

Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve

Biology of Reproduction

G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Accelerates Restoration of Ovarian Function in Cyclophosphamide … Pubmed: 31034039

International Journal of Reproductive Biomedicine

The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.

Reproductive Biology and Endocrinology

Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554

Andrologia

Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats Pubmed: 31353623

Journal of Ovarian Research

Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase Pubmed: 31722718

Genesis

Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterus Pubmed: 31815354

Chemosphere

Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling Pubmed: 31809938

Medicina

Effect of DHT-Induced Hyperandrogenism on the Pro-Inflammatory Cytokines in a Rat Model of Polycystic Ovary Morphology Pubmed: 32120970

Chemosphere

Prenatal exposure of female mice to perfluorononanoic acid delays pubertal activation of the reproductive endocrine axis through enhanced hepatic FGF21 … Pubmed: 33131727

BIOLOGICAL RESEARCH

Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance … Pubmed: 33109277

ARCHIVES OF GYNECOLOGY AND OBSTETRICS

MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance Pubmed: 32757043

Mol Med

Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic … Pubmed: 32698821

Antioxidants (Basel)

Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model Pubmed: 32517356

chemosphere

N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats Pubmed: 32447106

Neurotoxicology

Chronic exposure to low dose of bisphenol A causes follicular atresia by inhibiting kisspeptin neurons in anteroventral periventricular nucleus in female mice Pubmed: 32407858

BioMed Research International

Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress Pubmed: 32685488

ACS Applied Bio Materials

Evaluation for adverse effects of InP/ZnS quantum dots on the in vitro cultured oocytes of mice

Iranian Journal of Basic Medical Sciences

Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS

Medical Journal of Cairo University

Hemin Ameliorated Letrozole-Induced Polycystic Ovary Syndrome: A Forward Step for Better Management

biorxiv

The effects of human umbilical cord mesenchymal stem cell transplantation on female fertility preservation in mice


Drug-Herb Interaction Between Selective Estrogen Receptor Modulators and Estrogenic Herbal Medicine Er-Xian Decoction in Bone in Vivo and in Vitro

JOURNAL OF ETHNOPHARMACOLOGY

Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655

advanced biomedical research

The Effects of Zinc Methionine on Reproductive and Thyroid Hormones in Rats with Polycystic Ovarian Syndrome 33457340

Endocrinology

Diabetes Type 1 Negatively Influences Leydig Cell Function in Rats, Which is Partially Reversible By Insulin Treatment 33507237

JOURNAL OF ETHNOPHARMACOLOGY

BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose 33508368

Journal of Ethnopharmacology

Water extract of Er-xian decoction selectively exerts estrogenic activities and interacts with SERMs in estrogen-sensitive tissues 33823166

Toxicol Appl Pharmacol

Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion 33965371

researchsquare

Lipoamino Acid-Modified GnRH Analogs with Receptor-Mediated Antiproliferative Activity in Prostate and Ovarian Cancer Cells

Scientific reports

Regulation of Uterine Function During Estrous Cycle, Anestrus Phase and Pregnancy by Steroids in Red Deer (Cervus elaphus L.)


Chronic Exposure to Perfluorohexane Sulfonate Leads to a Reproduction Deficit by Suppressing Hypothalamic Kisspeptin Expression in Mice


Comparison of ovarian striation and ovarian fragmentation in a rat model of ovarian insufficiency

JOURNAL OF INHERITED METABOLIC DISEASE

Human and animal fertility studies in cystinosis reveal signs for obstructive azoospermia, a disruptive blood‐testis barrier and a subtherapeutic effect of cysteamine in … 34494673

Bioengineered

MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1 34637688

Free Radical Biology and Medicine

Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion 34610361

Prostate

Endothelial dysfunction after androgen deprivation therapy and the possible underlying mechanisms 34570375

Microbiome

Polyamine metabolism links gut microbiota and testicular dysfunction 34758869

J Virol

Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice 34730391

Stem Cells Int

Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … 34733331

Cell Death Dis

METTL3-mediated mRNA N6-methyladenosine is required for oocyte and follicle development in mice 34689175

Theriogenology

Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with … Pubmed:35063921

Nutrients

Yogurt Enriched with Inulin Ameliorated Reproductive Functions and Regulated Gut Microbiota in Dehydroepiandrosterone-Induced Polycystic Ovary Syndrome Mice Pubmed:35057459

Nat Commun

TrkB agonist antibody ameliorates fertility deficits in aged and cyclophosphamide-induced premature ovarian failure model mice Pubmed:35177657

Sci Total Environ

Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression Pubmed:35276145

Ecotoxicology and Environmental Safety

Constitutional delay of growth and puberty in female mice is induced by circadian rhythm disruption in utero Pubmed:35679725

Stem Cell Research & Therapy

G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and …

Research Square

Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency

BMC Veterinary Research

A promising animal model for Polycystic Ovary Syndrome: Borderline Hypertensive Rats


Catalog No.

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Applications (RESEARCH USE ONLY!)

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