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SKU:PAA991Hu01

Polyclonal Antibody to Ghrelin (GHRL)

Polyclonal Antibody to Ghrelin (GHRL)

Regular price $316.00 USD
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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;1:250-1000
;Immunohistochemistry: 5-20µg/mL;1:25-100
;Immunocytochemistry: 5-20µg/mL;1:25-100
;Optimal working dilutions must be determined by end user.

Product No.

PAA991Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA991Hu01-Recombinant Ghrelin (GHRL)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against GHRL. It has been selected for its ability to recognize GHRL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;1:250-1000
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Archives of Oral Biology

Expression of ghrelin in human salivary glands and its levels in saliva and serum in Chinese obese children and adolescents PubMed: 21075359

Department of Medical Biochemistry and Clinical Biochemistry

Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533

Journal of Physiology and Pharmacology

Postnatal administration of 2-oxoglutaric acid improves articular and growth plate cartilages and bone tissue morphology in pigs prenatally treated with dexamethasone PubMed: 23211309

Gene

The modifying effects of fish oil on fasting ghrelin mRNA expression in weaned rats PubMed: 22842192

Hormone Research in Paediatrics

Changes in Ghrelin and Obestatin Levels before and after a Meal in Children with Simple Obesity and Anorexia Pubmed: 23774845

Surgical Innovation

Gastric supply manipulation to modulate ghrelin production and enhance vascularization to the cardia: proof of the concept in a porcine model Pubmed:25294792

Obesity Surgery

Embolization of Arterial Gastric Supply in Obesity (EMBARGO): an Endovascular Approach in the Management of Morbid Obesity. Proof of the Concept in the Porcine Model Pubmed:25511751

Obes Surg

Effect of Sleeve Gastrectomy Plus Side-to-Side Jejunoileal Anastomosis for Type 2 Diabetes Control in an Obese Rat Model PubMed: 26202420

Surg Innov

Gastric Supply Manipulation to Modulate Ghrelin Production and Enhance Vascularization to the Cardia Proof of the Concept in a Porcine Model PubMed: 25294792

Nutrients. 

Elevation of Fasting Ghrelin in Healthy Human Subjects Consuming a High-Salt Diet: A Novel Mechanism of Obesity? Pubmed:27240398

World Journal of Gastroenterology

Effects of sleeve gastrectomy with jejuno-jejunal or jejuno-ileal loop onglycolipid metabolism in diabetic rats. pubmed:27621579

Ginekologia Polska

Increased serum ghrelin in preeclampsia: is ghrelin a friend or a foe? pubmed:27321099

Gynecological Endocrinology

The association between serum ghrelin levels and large-for-gestational-age fetuses in patients with gestational diabetes mellitus pubmed:27791436

Acta Diabetologica

Effect of lifestyle improvement program on the biomarkersof adiposity, inflammation and gut hormones in overweight/obese Asian Indians with prediabetes pubmed:28620678

Scandinavian Journal of Clinical and Laboratory Investigation

Role of serotonin hormone in weight regain after sleeve gastrectomy pubmed:29228802

Slovenian Veterinary Research

EFFECT OF Garcinia cambogia EXTRACT ON SERUM LEPTIN, GHRELIN, ADIPONECTIN AND INSULIN LEVELS AND BODY WEIGHTS IN RATS FED WITH HIGH … Slovetres:Source

The American Journal of the Medical Sciences

The Relations Among Serum Ghrelin, Motilin and Gastric Emptying and Autonomic Function in Autoimmune Gastritis Pubmed:29753372

American Journal of Perinatology

The Role of Umbilical Cord Blood Concentration of IGF-I, IGF-II, Leptin, Adiponectin, Ghrelin, Resistin, and Visfatin in Fetal Growth Pubmed: 30282106

Stem Cells and Development

The reparative effects of human adipose-derived mesenchymal stem cells in the chemotherapy damaged thymus Pubmed: 30511904

The Journal of Nutrition

Grape Seed Proanthocyanidin Affects Lipid Metabolism via Changing Gut Microflora and Enhancing Propionate Production in Weaned Pigs Pubmed: 31175811

Arch Physiol Biochem

Associations of apelin, leptin, irisin, ghrelin, insulin, glucose levels, and lipid parameters with physical activity during eight weeks of regular exercise training Pubmed: 31290696

Mol Med Rep

Ghrelin system is involved in improvements in glucose metabolism mediated by hyperbaric oxygen treatment in a streptozotocin‑induced type 1 diabetes mouse … Pubmed: 32901885

endocrine

Omega-3 offers better hypothalamus protection by decreasing POMC expression and elevating ghrelin hormone: a prospective trial to overcome methotrexate … Pubmed: 32424681

plos one

Serum leptin level is associated with phase angle in CKD5 patients not undergoing dialysis Pubmed: 30089154


Physiological and Histopathological Effects of Electroporation Pulse on Stomach of Rats

International Journal of Livestock Research

Effect of Nutritional Supplementation on Treatment of Anoestrus Swamp Buffalo Cows and Heifers

iraqi journal of science

Relationship Between Orexin-A and Insulin Resistance in Patients with Type 2 Diabetes Mellitus

Indian Journal of Forensic Medicine & Toxicology

Altered Serum Marker of Adipokines Profile in Breast Cancer Women

Br J Nutr

The Association of Pre-Pregnancy BMI On Leptin, Ghrelin, Adiponectin, and IGF-1 In Breast Milk, A Case Control Study 34348809

Nutrients

Nutritional Status, Selected Nutrients Intake and Their Relationship with the Concentration of Ghrelin and Adiponectin in Patients with Diabetic Nephropathy 34959967

scientific reports

Effect of Sleeve Gastrectomy, Roux-En-Y Gastric Bypass, and Ileal Transposition on Myocardial Ischaemia–Reperfusion Injury in Non-Obese Non-Diabetic Rats 34903800

npj Biofilms and Microbiomes

The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal Pubmed:35379849

Food Hydrocolloids

Creating texturally tuneable, low calorie and palatable noodle-like food assemblies via microfluidics


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