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SKU:PAB119Rb51

Polyclonal Antibody to Metallothionein 1 (MT1)

Polyclonal Antibody to Metallothionein 1 (MT1)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

Product No.

PAB119Rb51

Organism Species

Oryctolagus cuniculus (Rabbit).

Source

Polyclonal antibody preparation

Host

Guinea pig

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

APC

Immunogen

n/a

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a cavia polyclonal antibody raised against MT1. It has been selected for its ability to recognize MT1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Biotechology letters

Modulated expression of genes associated with NO signal transduction contributes to the cholesterol-lowering effect of electro-acupuncture SpringerLink: c1v4234150886817

International Journal of Diabetes in Developing Countries

Evaluation of serum metallothionein-1, selenium, zinc, and copper in Ghanaian type 2 diabetes mellitus patients Springer: Source

Experimental Oncology

Peculiarities of antioxidant system and iron metabolism in organism during development of tumor resistance to cisplatin Pubmed:25265354

Journal of Neurochemistry

Neuroprotective effects of levetiracetam target xCT in astrocytes in parkinsonian mice. Pubmed:26485447

Toxicology Mechanisms and Methods

Evaluation of hepatorenal impairments in Wistar rats coexposed to low-dose lead, cadmium and manganese: insights into oxidative stress mechanism pubmed:27599793

Environmental toxicology and pharmacology

Dyslipdemia induced by chronic low dose co-exposure to lead, cadmium and manganese in rats: the role of oxidative stress pubmed:28654832

European Journal of Immunology

Metallothionein‐1 suppresses rheumatoid arthritis pathogenesis by shifting the Th17/Treg balance Pubmed:30055006

Int Immunopharmacol

Metallothionein-1 is associated with osteoarthritis disease activity and suppresses proinflammatory cytokines production in synovial cells Pubmed: 31465913


Influence of cadmium on blood and hair trace elements and biochemical markers


Catalog No.

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