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SKU:PAB622Mu01

Polyclonal Antibody to Surfactant Protein B (SP-B)

Polyclonal Antibody to Surfactant Protein B (SP-B)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;
;Immunohistochemistry: 5-20µg/mL;
;Immunocytochemistry: 5-20µg/mL;
;Optimal working dilutions must be determined by end user.

Product No.

PAB622Mu01

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB622Mu01-Recombinant Surfactant Protein B (SP-B)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

1mg/ml

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SP-B. It has been selected for its ability to recognize SP-B in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Respiratory Physiology & Neurobiology

Kinetics of plasma SPB and RAGE during mechanical ventilation in patients undergoing major vascular surgery PubMed: 21736957

American Journal of Rhinology & Allergy

Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps PubMed: 23406594

19

Surfactant protein B and RAGE increases in the plasma during cardiopulmonary bypass: a pilot study PubMed: 20650982

Plos one

Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins PubMed: PMC3551896

Plos one

The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS PubMed: PMC3787032

Cancer Cell.

Lung Cancer Signatures in Plasma Based on Proteome Profiling of Mouse Tumor Models PubMed: PMC3406925

Respir Physiol Neurobiol

Acute high-altitude exposure reduces lung diffusion: Data from the HIGHCARE Alps project Pubmed: 23619193

PLOS ONE

Surfactant-Derived Proteins as Markers of Alveolar Membrane Damage in Heart Failure Pubmed:25514679


Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge Opus4:Source

Int J Cardiol

Plasma immature form of surfactant protein type B correlates with prognosis in patients with chronic heart failure. A pilot single-center prospective study PubMed: 26310985

Plos One

Serum Levels of Surfactant Proteins in Patients with Combined Pulmonary Fibrosis and Emphysema (CPFE) Pubmed:27337142

PLoS One

The Cerebral Surfactant System and Its Alteration in HydrocephalicConditions. pubmed:27656877

Frontiers in Aging Neuroscience

Correlations of Ventricular Enlargement with Rheologically Active SurfactantProteins in Cerebrospinal Fluid. pubmed:28101052

Frontiers in Aging Neuroscience

Correlations of Ventricular Enlargement with Rheologically Active Surfactant Proteins in Cerebrospinal Fluid PMC5209370

Respiratory Physiology & Neurobiology

Diving and pulmonary physiology: surfactant binding protein, lung fluid and cardiopulmonary test changes in professional divers pubmed:28467885

Cellular Physiology and Biochemistry

Surfactant Protein B Suppresses Lung Cancer Progression by Inhibiting Secretory Phospholipase A2 Activity and Arachidonic Acid Production pubmed:28743125

American Journal of Physiology. Lung Cellular and Molecular Physiology

Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. pubmed:29167126

Respiratory research

Effects and molecular mechanisms of intrauterine infection/inflammation on lung development Pubmed:29747649

Respiratory Physiology & Neurobiology 

Surfactant proteins changes after acute hemodynamic improvement in patients with advanced chronic heart failure treated with Levosimendan Pubmed:29548887

Lung 

Serum Surfactant Protein Levels in Patients Admitted to the Hospital with Acute COPD Exacerbation Pubmed:29445934

American Journal of Physiology Lung Cellular andMolecular Physiology

Generation of an alveolar epithelial type II cell line from induced pluripotent stem cells Pubmed: 30211653

Respiratory Research

Culture of human alveolar epithelial type II cells by sprouting Pubmed: 30340591


Catalog No.

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Applications (RESEARCH USE ONLY!)

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Magnetic Luminex Assay for Antigen Detection.

 

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