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SKU:PAF822Hu01

Polyclonal Antibody to Phosphatase And Tensin Homolog (PTEN)

Polyclonal Antibody to Phosphatase And Tensin Homolog (PTEN)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;1:300-1200
;Immunohistochemistry: 5-20µg/mL;1:30-120
;Immunocytochemistry: 5-20µg/mL;1:30-120
;Optimal working dilutions must be determined by end user.

Product No.

PAF822Hu01

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPF822Hu01-Recombinant Phosphatase And Tensin Homolog (PTEN)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

0.5mg/ml

Organism Species More

Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Equus caballus; Equine (Horse), Chicken (Gallus)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PTEN. It has been selected for its ability to recognize PTEN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Experimental and Therapeutic Medicine

Elucidating the mechanism of miRNA‑214 in the regulation of gingival carcinoma 10.3892/etm.2017.4264

Cancers (Basel)

In Vivo and In Vitro Enhanced Tumoricidal Effects of Metformin, Active Vitamin D3, and 5-Fluorouracil Triple Therapy against Colon Cancer by Modulating the … Pubmed:35326689


Metformin and Calcitriol Enhance 5-Fluorouracil Tumoricidal Effects in Colon Cancer By Modulating The PI3K/Akt/PTEN/mTOR Network


Catalog No.

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Applications (RESEARCH USE ONLY!)

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ELISA Kit DIY Materials for Phosphatase And Tensin Homolog (PTEN)

Main materials for "Do It(ELISA Kit) Yourself"

 

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