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SKU:PAB972Mu02

Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF)

Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAB972Mu02

Organism Species

Mus musculus (Mouse).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

n/a

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PEDF. It has been selected for its ability to recognize PEDF in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Molecular Therapy

A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions Nature: 2010144

American Journal of Nephrology

Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy Karger: 314326

34

Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury OxfordJournals: source

Heart and Education in Heart

The change of serum level of pigment epithelium-derived factor in coronary heart disease Bmj: Source

Mediators of Inflammation

Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation Pubmed: 24288442

Cardiology.

The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. Pubmed: 24192856

Endocrinology

Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism Endocrine: Source

Clinical Science

Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis PubMed: 25700221

Molecular Sciences

Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R Pubmed:27187377

Investigative ophthalmoloey & visual science

The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells articleid:2582822

Scientific reports

VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice pubmed:28091556

国际眼科杂志

色素上皮衍生因子修饰的人脐带间充质干细胞构建方法 201707007

Investigative Ophthalmology & Visual Science

PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice Pubmed:30025136

International Eye Science

高海拔地区新生血管性青光眼不同治疗方式的疗效及对房水中PEDF, VEGF 水平的影响 2018/2/201802020.pdf

Domestic Animal Endocrinology

Lactation-related changes in tissue expression of PEDF in dairy cows Pubmed:29758402

Medical Science Monitor

Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a … Pubmed:29574467

Chemosphere

Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis Doi: 10.1016/j.chemosphere.2018.10.100

Biological Trace Element Research

Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model Pubmed: 30392020

International Urology and Nephrology

Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement Pubmed: 30536192

International Journal of Ophthalmology

A novel xeno-free culture system for human retinal pigment epithelium cells Pubmed: 31024807

Journal of the American Heart Association

Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia Pubmed: 31711388

environmental research

Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells Pubmed: 32315827

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells 33498962

Hum Cell

Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ¡­ 33768511


Catalog No.

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