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SKU:PAA009Ra02

Polyclonal Antibody to Angiopoietin 2 (ANGPT2)

Polyclonal Antibody to Angiopoietin 2 (ANGPT2)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Product No.

PAA009Ra02

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPA009Ra02-Recombinant Angiopoietin 2 (ANGPT2)

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Homo sapiens (Human), Mus musculus (Mouse)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ANGPT2. It has been selected for its ability to recognize ANGPT2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Microvascular Research

Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289

Theriogenology

Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes Science: Article

Pediatr Infect Dis J

Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean–Congo Hemorrhagic Fever PubMed: 25831422

Oncotarget

Long noncoding RNA hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways Pubmed:26717040

Experimental Animals

Ginsenoside Rb1 improves cardiac function and remodeling in heart failure. pubmed:28367863

Sci Rep. 

Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. pubmed:28276491

PeerJ 

The ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker pubmed:28584715

Cellular Physiology and Biochemistry

Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats pubmed:28848201

Tumor Biology

Cervical cancer cell–derived angiopoietins promote tumor progression pubmed:28720059

Anesthesiology

Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … Pubmed:28968277

British Journal of Anaesthesia

Vasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats 10.1016:j.bja.2018.05.049

Anticancer Research

Oligodendrocytes Up-regulate the Invasive Activity of Glioblastoma Cells via the Angiopoietin-2 Signaling Pathway Pubmed: 30711932

Comparative Medicine

Characterization of a Porcine Model for Von Willebrand Disease Type 1 and 3 Regarding Expression of Angiogenic Mediators in the Nonpregnant Female … Pubmed: 31526432

BMC Endocrine Disorders

The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children Pubmed: 31771561

Biology of Reproduction

Expression of angiogenic factors in the uteroplacental unit is altered at time of placentation in a porcine model of von Willebrand disease type 1 Pubmed: 31806575

Scientific Reports

Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea Pubmed: 31882827

MEDIATORS OF INFLAMMATION

Mica Can Alleviate TNBS-Induced Colitis in Mice by Reducing Angiotensin II and IL-17A and Increasing Angiotensin-Converting Enzyme 2, Angiotensin 1-7 … Pubmed: 33100902

Scientific Reports

Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats Pubmed: 32616735

Intensive Care Med Exp

The effect of targeting Tie2 on hemorrhagic shock-induced renal perfusion disturbances in rats 33997943

Gynecol Endocrinol

Potential of thrombospondin-1 in treatment of polycystic ovary syndrome rat model: a preliminary study 34282706

Clinical and Experimental Immunology

Clinical significance of differential serum-signatures for early prediction of severe dengue among Eastern Indian patients Pubmed:35348620


Catalog No.

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