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SKU:PAA106Hu02
Polyclonal Antibody to Neurotrophin 3 (NT3)
Polyclonal Antibody to Neurotrophin 3 (NT3)
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Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.
Product No.
PAA106Hu02
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA106Hu02-Recombinant Neurotrophin 3 (NT3)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NT3. It has been selected for its ability to recognize NT3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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S |
Caracteriza Teses: Disponiveis |
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Canadian Journal of Physiology and Pharmacology |
Protective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factor pubmed:28388365 |
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Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso : |
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Artificial Cells Nanomedicine and Biotechnology |
Histological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered grafts Pubmed: 31899965 |
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laboratory investigation |
Macrophage-derived neurotrophin-3 promotes heterotopic ossification in rats Pubmed: 31896816 |
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EUROPEAN CHILD & ADOLESCENT PSYCHIATRY |
Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158 |
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BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY |
Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways 33589896 |
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Clin Epigenetics |
Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism 34092241 |
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J Nutr Sci Vitaminol (Tokyo) |
Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice 34193676 |
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Iranian Journal of Basic Medical Sciences |
Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons 34712429 |
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Molecular Biology Reports |
Umbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling … Pubmed:35585377 |
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