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SKU:PAB807Hu02

Polyclonal Antibody to Jagged 1 (JAG1)

Polyclonal Antibody to Jagged 1 (JAG1)

Regular price $332.00 USD
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UOM
Concentration
Organism species

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

Product No.

PAB807Hu02

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB807Hu02-Recombinant Jagged 1 (JAG1)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Mus musculus (Mouse), Rattus norvegicus (Rat)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against JAG1. It has been selected for its ability to recognize JAG1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

13

Cross-talk between the Toll-like receptor 4 and Notch1 pathways augments the inflammatory response in the interstitial cells of stenotic human aortic valves PubMed: PMC3506187

Eur J Obstet Gynecol Reprod Biol.

Profiling of selected angiogenesis-related genes in proliferative eutopic endometrium of women with endometriosis Pubmed: 24188612

PLoS One.

Augmented Osteogenic Responses in Human Aortic Valve Cells Exposed to oxLDL and TLR4 Agonist: A Mechanistic Role of Notch1 and NF-κB Interaction Pubmed:Pmc4014478

Biochim Biophys Acta.?

Ligation of ICAM-1 on human aortic valve interstitial cells induces the osteogenic response: A critical role of the Notch1-NF-κB pathway in BMP-2 expression Pubmed:25101972

international journal of oral-medical sciences

Jagged1 Stimulates Bone Resorption during Orthodontic Tooth Movement Dn: Jlc

Oncology letters

MicroRNA‑26b acts as an antioncogene and prognostic factor in cervical cancer

Journal of Biological Chemistry

Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328

Cell Death & Disease

Growth hormone induces mitotic catastrophe of glomerular podocytes and contributes to proteinuria 33795655


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