Polyclonal Antibody to Ki-67 Protein (Ki-67)

Western blotting: 0.5-2µg/mL;1:300-1200
;Immunohistochemistry: 5-20µg/mL;1:30-120
;Immunocytochemistry: 5-20µg/mL;1:30-120
;Optimal working dilutions must be determined by end user.

Product No.

PAC047Hu02

Organism Species

Homo sapiens (Human).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPC047Hu02-Recombinant Ki-67 Protein (Ki-67)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

0.58mg/ml

Organism Species More

Mus musculus (Mouse)

Applications

WB; ICC; IHC-P; IHC-F; ELISA.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against Ki-67. It has been selected for its ability to recognize Ki-67 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL;1:300-1200
Immunohistochemistry: 5-20µg/mL;1:30-120
Immunocytochemistry: 5-20µg/mL;1:30-120
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.