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SKU:PAB226Ra02

Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)

Polyclonal Antibody to Corticosteroid Binding Globulin (CBG)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAB226Ra02

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPB226Ra02-Recombinant Corticosteroid Binding Globulin (CBG)

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CBG. It has been selected for its ability to recognize CBG in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

General Endocrinology

The Regulation of Rasd1 Expression by Glucocorticoids and Prolactin Controls Peripartum Maternal Insulin Secretion Endjournals: Source

PLoS One

Modulation in Wistar Rats of Blood Corticosterone Compartmentation by Sex and a Cafeteria Diet PubMed: PMC3579843

Annals of Anatomy

Differences in colocalization of corticosteroid-binding globulin and glucocorticoid receptor immunoreactivity in the rat brain ScienceDirect: S0940960212001628

Physiological Reports

Adrenocortical control in the neonatal rat: ACTH‐and cAMP‐independent corticosterone production during hypoxia Pubmed: 24303136

Shock.

Adrenocorticotropic Hormone But Not High-Density Lipoprotein Cholesterol or Salivary Cortisol Was a Predictor of Adrenal Insufficiency in Patients With Septic Shock. Pubmed: 24667620

Neurobiology of Aging

Greater glucocorticoid receptor activation in hippocampus of aged rats sensitizes microglia Pubmed:25559333

Cell Death Differ.

Novel p53 target genes secreted by the liver are involved in non-cell-autonomous reCavia (Guinea pig )lation Pubmed:26358154

Journal of Exercise, Sports & Orthopedics

Impact of Season and Oral Contraceptive use on Cortisol Levelsin Physically Active Women exercise-sports-orthopedics:exercise-sports-orthop


Impact of Season and Oral Contraceptive use on Cortisol Levels in Physically Active Women diva2:1047030

Neuropharmacology

Impaired glucocorticoid-mediated HPA axis negative feedback induced by juvenile social isolation in male rats Pubmed:29407214

Pharmacological Reports

The impact of early-life stress on corticosteroid carrier protein levels and 11β-hydroxysteroid dehydrogenase 1 expression in adolescent rats


Catalog No.

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