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SKU:PAC011Ra02

Polyclonal Antibody to Complement Factor B (CFB)

Polyclonal Antibody to Complement Factor B (CFB)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

PAC011Ra02

Organism Species

Rattus norvegicus (Rat).

Source

Polyclonal antibody preparation

Host

Rabbit

Potency

n/a

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

None

Immunogen

RPC011Ra02-Recombinant Complement Factor B (CFB)

Buffer Formulation

PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

Mus musculus (Mouse)

Applications

WB; IHC; ICC; IP.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CFB. It has been selected for its ability to recognize CFB in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

BMC Nephrology

Complement in patients receiving maintenance hemodialysis: functional screening and quantitative analysis BioMed: 14712369

J. Proteome Res.

Identification and Confirmation of Differentially Expressed Fucosylated Glycoproteins in the Serum of Ovarian Cancer Patients Using a Lectin Array and LC–MS/MS ACS: pr300330z

Pediatr Int

Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527

Journal of Neuroimmunology

Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358

Biochemical and Biophysical Research Communications

Adipose tissue complement factor B promotes adipocyte maturation. pubmed:29137982

Journal of proteome research

Serum exosomes from newborn piglets restrict porcine epidemic diarrhea virus infection

journal of human genetics

Identification of susceptibility locus shared by IgA nephropathy and inflammatory bowel disease in a Chinese Han population Pubmed: 31857673

Nephrol Dial Transplant

Association between thrombotic microangiopathy and activated alternative complement pathway in malignant nephrosclerosis 33367879

JOURNAL OF GENERAL VIROLOGY

Changes in complement alternative pathway components, factor B and factor H during dengue virus infection in the AG129 mouse 33410734

J Proteome Res

Early Diagnostic Ability of Human Complement Factor B in Pancreatic Cancer Is Partly Linked to Its Potential Tumor-Promoting Role 34766501

J Clin Med

A Three-Protein Panel to Support the Diagnosis of Sepsis in Children Pubmed:35329889


Catalog No.

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Applications (RESEARCH USE ONLY!)

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