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SKU:PAB785Hu02
Polyclonal Antibody to Neprilysin (CD10)
Polyclonal Antibody to Neprilysin (CD10)
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Western blotting: 0.5-5µg/mL
;Immunocytochemistry in formalin fixed cells: 5-20µg/mL
;Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
;Immunohistochemistry in paraffin section: 5-20µg/mL
;Optimal working dilutions must be determined by end user.
Product No.
PAB785Hu02
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPB785Hu02-Recombinant Neprilysin (CD10)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Mus musculus (Mouse), Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CD10. It has been selected for its ability to recognize CD10 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-5µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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13 |
Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme Inhibitors Ahajournals:Source |
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Biol Pharm Bull |
Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty Rats PubMed: 25832640 |
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Eur J Heart Fail. |
Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fraction Pubmed:26725876 |
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8 |
A Test in Context: Neprilysin: Function, Inhibition, and Biomarker. pubmed:27491909 |
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European Journal of Heart Failure |
Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study Pubmed: 30520545 |
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European Journal of Heart Failure |
Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failure Pubmed: 30828920 |
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Clinical Research in Cardiology |
Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD) Pubmed: 32002632 |
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Neurotoxicology |
Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice Pubmed: 32522471 |
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European Heart Journal |
P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction |
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Clin Biochem |
Finding a reliable assay for soluble neprilysin Pubmed:35331754 |
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Scientific Reports |
The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in … |
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Catalog No. |
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