Cloud-clone
SKU:PAA868Hu04
Polyclonal Antibody to Endothelial NOS (eNOS)
Polyclonal Antibody to Endothelial NOS (eNOS)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
PAA868Hu04
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA868Hu04-Recombinant Endothelial NOS (eNOS)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against eNOS. It has been selected for its ability to recognize eNOS in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Journal of Ethnopharmacology |
Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells ScienceDirect: S0378874109004541 |
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PLoS One |
A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide PubMed: 23359814 |
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6th International Scientific Interdisciplinary Conference (ISIC) |
Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity Knmu: Source |
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PLoS One. |
SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases Plos: Source |
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INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH |
L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION Tdmu:Source |
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Journal of Clinical Laboratory Analysis |
Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome Pubmed:24798146 |
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Endocrine |
Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia Pubmed:24840719 |
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PLOS ONE |
A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: PubMed: 25785999 |
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J Trace Elem Med Biol |
Comparative angioprotective effects of magnesium compounds PubMed: 25127069 |
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Journal of Functional Foods |
Peptides from hydrolysate of lantern fish (Benthosema pterotum) proved neuroprotective in vitro and in vivo science:S1756464616300639 |
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Медицина и здравоохранение |
Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу article:n |
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journal of molecular and cellular cardiology |
Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. pubmed:28088561 |
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International Journal of Medicine and Medical Research |
ThE REmOdELINg Of LEfT ATRIUm ANd vAsOdILATION fAcTORs chANgEs IN IschEmIc hEART fAILURE 7038 |
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International Journal of Ophthalmology |
Endothelial nitric oxide synthase deficiency influences normal cell cycle progression andapoptosis in trabecular meshwork cells. pubmed:27366677 |
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Biomed Pharmacother. |
SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. pubmed:28122311 |
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Biomedicine & Pharmacotherapy |
SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death DOI:10.1016/j.biopha.2017.01.087 |
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Asian Journal of Science and Technology |
THE INFLUENCE OF THE ADDITIONAL USAGE OF L-ARGININE ON THE FACTORS OF VASODILATION AND THE CLINICAL DATA, COMPLICATED WITH CARDIAC DECOMPENSATION WITH REDUCED AND PRESERVED FRACTURE OF THE LEFT VENTRICLE THAT PATIENTS WITH STABLE CORONARY HEART DISEASE HAVE ISSN: 0976-3376 |
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National Science Review |
Blunted Nitric Oxide Regulation in Tibetans under High Altitude Hypoxia 10.1093:nsr |
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Biomedicine & Pharmacotherapy |
Molecular basis of vascular damage caused by cigarette smoke exposure and a new approach to the treatment: Alpha-linolenic acid Pubmed:29579706 |
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Experimental and Therapeutic Medicine |
Ergothioneine prevents endothelial dysfunction induced by mercury chloride Pubmed:29805489 |
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Turk Kardiyol Dern Ars. |
Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarction Pubmed:29664427 |
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Stężenia śródbłonkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ciężarnych palących tytoń–badania wstępne |
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The Eurasian Journal of Medicine |
Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats Doi: 10.5152/eurasianjmed.2018.18160 |
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International Journal of Environmental Research and Public Health |
Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women Pubmed: 30513890 |
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Journal of Physics D: Applied Physics |
Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility |
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Particle and Fibre Toxicology |
Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway Pubmed: 30975181 |
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ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ … |
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Динамика показателей эндотелиальной дисфункции до и после операции у больных варикозной болезнью нижних конечностей |
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European Journal of Pharmacology |
The placental growth factor attenuates intimal hyperplasia in vein grafts by improving endothelial dysfunction Pubmed: 31836533 |
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Rheumatol Int |
Circadian fluctuations of endothelial nitric oxide synthase activity in females with rheumatoid arthritis: a pilot study Pubmed: 32025851 |
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Reviews on Recent Clinical Trials |
Circadian rhythms of endothelial nitric oxide synthase and toll-like receptors 2 production in females with rheumatoid arthritis depending on NOS3 gene polymorphism … Pubmed: 32297585 |
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Georgian medical news |
ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE … Pubmed: 32242853 |
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Zaporozhye medical journal |
Dynamics of endothelial dysfunction indices before and after operation in patients with varicose disease of the lower extremities |
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regional hemodynamics and microcirculation |
Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility |
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Оксидативный стресс и эндотелиальная дисфункция в развитии экспериментального радиоиндуцированного повреждения легких и их коррекция … |
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Reprod Biol |
Angiogenic factors measured in aspirated placental tissue between the 10+ 6 and 18+ 3 weeks of gestation 34798517 |
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J Basic Clin Physiol Pharmacol |
Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats Pubmed:34986543 |
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Eur Rev Med Pharmacol Sci |
Serum inducible and endothelial nitric oxide synthase in coronary artery disease patients with Type 2 Diabetes mellitus Pubmed:35647851 |
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Evidence-Based Complementary and Alternative Medicine |
Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory … Pubmed:35620404 |
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