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SKU:LAB409Bo71
Biotin-Linked Polyclonal Antibody to Hemoglobin (HB)
Biotin-Linked Polyclonal Antibody to Hemoglobin (HB)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB409Bo71
Organism Species
Bos taurus; Bovine (Cattle).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HB. It has been selected for its ability to recognize HB in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology |
Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna Pubmed:25460046 |
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PLoS One. |
Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis Pubmed:27027307 |
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Parasites & Vectors |
The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. pubmed:27561965 |
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Proteomics. Clinical Applications |
Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. pubmed:27615121 |
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Molecular nutrition & food research |
Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro pubmed:28105758 |
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Frontiers in Immunology |
Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus pubmed:28694810 |
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新規人工酸素運搬体HemoAct を用いた脳虚血再灌流障害治療開発に関する研究 : |
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Acta Neuropathologica Communications |
Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke Pubmed: 30777135 |
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Angiogenesis |
Decylubiquinone suppresses breast cancer growth and metastasis by inhibiting angiogenesis via the ROS/p53/BAI1 signaling pathway Pubmed: 32020421 |
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Food Bioscience |
Water-extractable zinc protoporphyrin IX in Parma ham predominantly exists as complexes with hemoglobin and myoglobin |
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Photochem Photobiol |
Effects of Human Serum Albumin on the Fluorescence Intensity and Tumor Imaging Properties of IR‐780 Dye 34687567 |
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Catalog No. |
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