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SKU:PAA627Hu02
Polyclonal Antibody to Caspase 9 (CASP9)
Polyclonal Antibody to Caspase 9 (CASP9)
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Western blotting: 1-5ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
PAA627Hu02
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPA627Hu02-Recombinant Caspase 9 (CASP9)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CASP9. It has been selected for its ability to recognize CASP9 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Reprod Sci. |
Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells. Pubmed: 23420823 |
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Molecular Neurobiology |
Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations Pubmed:25502462 |
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BMC Cancer |
Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells. pubmed:27461108 |
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Exp Lung Res |
Stretch-induced apoptosis in rat alveolar epithelial cells is mediated by the intrinsic mitochondrial pathway. pubmed:28394655 |
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The American Journal of Chinese Medicine |
Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells pubmed:28231747 |
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Frontiers in Pharmacology |
Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus. pubmed:28553224 |
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Clinical And Experimental Pharmacology And Physiology |
Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway Pubmed:29266336 |
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Environmental Toxicology |
Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice Pubmed: 31087429 |
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European journal of medicinal chemistry |
Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity Pubmed: 31216504 |
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Odontology |
Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337 |
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Environ Sci Pollut Res Int |
Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of … Pubmed: 32915453 |
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SAGE Journals |
Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic … |
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Eur J Pharmacol |
Potential cardioprotective effect of octreotide via NOXs mitigation, mitochondrial biogenesis and MAPK/Erk1/2/STAT3/NF-kβ pathway attenuation in isoproterenol … Pubmed:35500641 |
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