Cloud-clone
SKU:LAA388Ra81
FITC-Linked Polyclonal Antibody to Complement Component 5a (C5a)
FITC-Linked Polyclonal Antibody to Complement Component 5a (C5a)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA388Ra81
Organism Species
Rattus norvegicus (Rat).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against C5a. It has been selected for its ability to recognize C5a in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Molecular Immunology |
Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297 |
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Journal of Reproductive Immunology |
Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845 |
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The Journal of Clinical Investigation |
Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471 |
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Journal of Antimicrobial Chemotherapy |
Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587 |
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Journal of Orthopaedic Research |
Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742 |
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SpringerPlus |
Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977 |
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American Journal fo Reproductive Immunology |
Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155 |
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Gynecol Obstet Invest |
Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247 |
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Journal of Neuroimmunology |
Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358 |
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International Immunopharmacology |
Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602 |
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Digital Repository |
Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183 |
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Environmental Pollution |
Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439 |
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Cancer Letters |
Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586 |
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Acta Paediatrica |
Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106 |
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Scientific Reports |
Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296 |
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Nutrients |
The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479 |
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Journal of NeuroVirology |
Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976 |
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PLoS One |
Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004 |
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Frontiers in Immunology |
C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855 |
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Phytomedicine |
Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 … |
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Aging |
C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478 |
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STEM CELL REVIEWS AND REPORTS |
A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006 |
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Modulation of C5a-C5aR interactions against murine mammary cancer cell line |
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FASEB J |
C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088 |
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