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SKU:LAA297Ra81

FITC-Linked Polyclonal Antibody to Ribonuclease A (RNase A)

FITC-Linked Polyclonal Antibody to Ribonuclease A (RNase A)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA297Ra81

Organism Species

Rattus norvegicus (Rat).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

FITC

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC; IF.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against RNase A. It has been selected for its ability to recognize RNase A in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

International Journal of Molecular Sciences

The Human Host Defense Ribonucleases 1, 3 and 7 Are Elevated in Patients with Sepsis after Major Surgery—A Pilot Study Pubmed:26927088

Investigative Ophthalmology & Visual Science

The Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal Disease Pubmed:30025106

Nat Commun

Human ribonuclease 1 serves as a secretory ligand of ephrin A4 receptor and induces breast tumor initiation 33986289


Catalog No.

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Applications (RESEARCH USE ONLY!)

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LAA297Ra81

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Biotin-Linked Polyclonal Antibody to Ribonuclease A (RNase A)

WB; IHC; ICC.

 

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