Cloud-clone
SKU:LAA899Ca71
Biotin-Linked Polyclonal Antibody to Osteopontin (OPN)
Biotin-Linked Polyclonal Antibody to Osteopontin (OPN)
Couldn't load pickup availability
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA899Ca71
Organism Species
Canis familiaris; Canine (Dog).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against OPN. It has been selected for its ability to recognize OPN in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|
Magazine |
Citations |
|
Journal of Biomedical Materials Research Part A |
Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture PubMed: 19280635 |
|
Osteoarthritis and Cartilage |
Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients PubMed: 19747583 |
|
The Journal of Rheumatology |
Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis. Jrheum: source |
|
International Journal of Pharmaceutics |
Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester) ScienceDirect: S0378517312003742 |
|
European Cells and matreials |
The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering Ecmjournal: Source |
|
Journal of Equine Veterinary Science |
Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse ScienceDirect: Source |
|
Basic & Clinical Pharmacology & Toxicology |
Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions Wiley:Source |
|
Journal of Cellular Biochemistry |
Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts Pubmed:24905552 |
|
Naunyn-Schmiedeberg's Archives of Pharmacology |
Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats Pubmed:25038619 |
|
J Endocrinol Invest.? |
The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders Pubmed:25245337 |
|
FEBS Letters |
Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells Pubmed:25542352 |
|
Gene |
Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis PubMed: 26283150 |
|
Medicine (Baltimore) |
An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer PubMed: 26266370 |
|
Journal of Biomaterials and Tissue Engineering |
Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel Content: Asp |
|
Primasari |
Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer Index.Php: Amor |
|
Int J Biol Sci |
Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway PubMed: 26327817 |
|
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao |
Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma. Ebscohost |
|
Am J Pathol. |
Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy. Pubmed:26963343 |
|
circulation journal |
Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification. pubmed:28344201 |
|
Journal of Cardiovascular Electrophysiology |
Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure. pubmed:28127817 |
|
American Journal of Translational Research |
Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes. pubmed:28337262 |
|
Diabetes Care |
Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans pubmed:29212824 |
|
Biomedicine & pharmacotherapy |
Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation. pubmed:28742995 |
|
Journal of Alzheimers Disease |
Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods Pubmed:30040720 |
|
Indian Journal of Nephrology |
Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease id:233806 |
|
Carbon |
In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells Doi: 10.1016/j.carbon.2018.10.081 |
|
current eye research |
Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma. Pubmed: 30994369 |
|
Journal of Proteomics |
Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma Pubmed: 31629959 |
|
Acta Biomaterialia |
Inflammatory response to magnesium-based biodegradable implant materials Pubmed: 31610341 |
|
Journal of Clinical Medicine |
Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and … Pubmed: 32182704 |
|
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES |
Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats Pubmed: 33010420 |
|
[ENGINEERING OF BIOMATERIALS |
Osteoconductive potential of PLGA/bioglass composite biomaterials |
|
Biomolecules |
Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44 34070790 |
|
Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells |
|
|
Parasit Vectors |
Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate … 34857049 |
|
Medicina (Kaunas) |
Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study Pubmed:35743980 |
|
Catalog No. |
Related products for research use of Canis familiaris; Canine (Dog) Organism species |
Applications (RESEARCH USE ONLY!) |
|
RPA899Ca01 |
Positive Control; Immunogen; SDS-PAGE; WB. |
|
|
RPA899Ca02 |
Positive Control; Immunogen; SDS-PAGE; WB. |
|
|
PAA899Ca01 |
WB; IHC; ICC; IP. |
|
|
LAA899Ca71 |
WB; IHC; ICC. |
|
|
HEA899Ca |
Enzyme-linked immunosorbent assay for Antigen Detection. |
|
|
SEA899Ca |
Enzyme-linked immunosorbent assay for Antigen Detection. |
|
|
LMA899Ca |
Magnetic Luminex Assay for Antigen Detection. |
|
|
KSA899Ca01 |
Main materials for "Do It (ELISA Kit) Yourself". |
Share
