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SKU:LAA518Hu71

Biotin-Linked Polyclonal Antibody to Ferritin (FE)

Biotin-Linked Polyclonal Antibody to Ferritin (FE)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA518Hu71

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against FE. It has been selected for its ability to recognize FE in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Molecular Endocrinology

PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. Pubmed: 23438894

experimental oncology

Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. PubMed: 25804229

J Cancer Sci Ther

Host H68D Genotype Affects Tumor Growth in Mouse Melanoma Open-Access: 1948-5956-1000353

Food Funct

The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice Pubmed:26757699

Physiological research

The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease pubmed:27782743

Expert Review of Hematology

4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. pubmed:27615544

Oxidative Medicine and Cellular Longevity

Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation pubmed:29147463

European Journal of Pharmaceutical Sciences

sucrosomial® iron absorption studied by in vitro and ex-vivo models pubmed:29055735

Journal of Food Science and Technology

In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. pubmed:29051659

International Journal of Cuban Health & Medicine

Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women 10.1590/MEDICC.2017.1902030004 

Oman Medical Journal

The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. pubmed:28584599

Food Bioscience

Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system 10.1016/j.fbio.2017.10.001

Nutrients.

Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats pubmed:28629118

Food Chemistry

Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells Pubmed:29622185

Animal Feed Science and Technology

Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet 10.1016:j.anifeedsci.2018.01.012

American Journal of Hematology

Iron homeostasis in pregnancy and spontaneous abortion Pubmed: 30394565

The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria

Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria

Haematologica

New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia Pubmed: 30792208

博士论文

PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE


Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic

Cell Research

Transferrin plays a central role in coagulation balance by interacting with clotting factors Pubmed: 31811276

international journal of biological macromolecules

Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications Pubmed: 31904465

journal of the american college of nutrition

Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation Pubmed: 32255407

LWT-FOOD SCIENCE AND TECHNOLOGY

Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability

J Mol Cell Cardiol

Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure 34139233

European Journal of Pharmacology

The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 Pubmed:35700835


Catalog No.

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