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SKU:LAA082Hu71

Biotin-Linked Polyclonal Antibody to Laminin (LN)

Biotin-Linked Polyclonal Antibody to Laminin (LN)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAA082Hu71

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LN. It has been selected for its ability to recognize LN in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Stem Cell Research Therapy

Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658

PLoS One.

Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592

Clin Exp Metastasis.

Augmented expression of urokinase plasminogen activator and extracellular matrix proteins associates with multiple myeloma progression Pubmed:24807734

European Journal of Obstetrics & Gynecology and Reproductive Biology

Investigation?of?diagnostic?potentials?of?nine?different?biomarkers?in?endometriosis. Pubmed:24813083

Archives of Plastic Surgery

Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas PubMed: 26430624

Canadian Journal of Physiology and Pharmacology

Modified Citrus Pectin stops progression of liver fibrosis by inhibiting galactin-3 and inducing apoptosis of stellate cells Doi: Abs

MOLECULAR AND CLINICAL ONCOLOGY

Clinical significance of serum laminin and typeâIV collagen levels in cutaneous melanoma patients Pubmed:27330797

Am J Transplant.

Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. pubmed:27456745

Asian Pacific Journal of Cancer Prevention

Modulatory Effects of Chemoradiation on Angiogenic Factors and Laminin in Cervical Cancer: Link with Treatment Response pubmed:29172262

Diabetes

Coordination Among Lipid Droplets, Peroxisomes and Mitochondria Regulates Energy Expenditure Through the CIDE-ATGL-PPARα Pathway in Adipocytes Pubmed:29986925

European Food Research and Technology

Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis 10.1007:s00217-018-3119-z

Experimental Lung Research

High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects Pubmed: 31290711

Neurotherapeutics

Fabrication and Evaluation of a Xenogeneic Decellularized Nerve-Derived Material: Preclinical Studies of a New Strategy for Nerve Repair Pubmed: 31758411


Untersuchungen und semiquantitative Symmetrie-Analysen zur Extrazellulärmatrix von Kalbs-Stimmlippen

ARCHIV?EUROMEDICA

CLINICAL SIGNIFICANCE OF LAMININ AND ELASTINE LEVELS IN CHILDREN WITH UNDIFFERENTIATED CONNECTIVE TISSUE DISEASE


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