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SKU:LAA705Hu71
Biotin-Linked Polyclonal Antibody to Mucin 2 (MUC2)
Biotin-Linked Polyclonal Antibody to Mucin 2 (MUC2)
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Western blotting: 0.5-2ug/ml
;Immunocytochemistry in formalin fixed cells: 5-20ug/ml
;Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
;Immunohistochemistry in paraffin section: 5-20ug/ml
;Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
;Optimal working dilutions must be determined by end user.
Product No.
LAA705Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
PAA705Hu01-Polyclonal Antibody to Mucin 2 (MUC2)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MUC2. It has been selected for its ability to recognize MUC2 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Annals of surgical oncology |
Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas. PubMed: 20717734 |
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World J Gastroenterol. |
Sodium alginate ameliorates indomethacin-induced gastrointestinal mucosal injury via inhibiting translocation in rats. Pubmed:24627600 |
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Front Microbiol |
Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis PubMed: 26217323 |
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nutrition research and practice |
Butyrate modulates bacterial adherence on LS174T human colorectal cells by stimulating mucin secretion and MAPK signaling pathway PubMed: 26244071 |
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Respirology |
Secreted mucins and airway bacterial colonization in non-CF bronchiectasis PubMed: 26172851 |
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Ann Am Thorac Soc |
Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization doi:10.1513 |
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Cellular Microbiology |
An adherent mucus layer attenuates the genotoxic effect of colibactin. pubmed:29156489 |
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Bio-protocol |
Enhancement of Mucus Production in Eukaryotic Cells and Quantification of Adherent Mucus by ELISA 10.21769:BioProtoc.2879 |
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Journal of Investigative Medicine |
The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease Pubmed: 30573494 |
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Biomolecules |
Clinical Significance of Serum Membrane-Bound Mucin-2 Levels in Breast Cancer Pubmed: 30682816 |
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Cell Stem Cell |
PRDM16 Maintains Homeostasis of the Intestinal Epithelium by Controlling Region-Specific Metabolism Pubmed: 31564549 |
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DIGESTIVE DISEASES AND SCIENCES |
Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats Pubmed: 32144600 |
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Scientific Reports |
Mucus layer modeling of human colonoids during infection with enteroaggragative E. coli Pubmed: 32601325 |
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Biorxiv |
Defective intestinal repair after short-term high fat diet due to loss of efferocytosis |
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J Cell Mol Med |
Inhibition of cyclooxygenase©\2 enhanced intestinal epithelial homeostasis via suppressing ¦Â©\catenin signalling pathway in experimental liver fibrosis 34145945 |
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Cell Tissue Res |
Reduced acetylcholine and elevated muscarinic receptor 2 in duodenal mucosa contribute to the impairment of mucus secretion in 6-hydroxydopamine-induced … 34370080 |
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