Cloud-clone
SKU:LAB697Hu71
Biotin-Linked Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT)
Biotin-Linked Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB697Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against a1AT. It has been selected for its ability to recognize a1AT in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Journal of Biotechnology |
Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed PubMed: 23376844 |
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Sci Rep |
Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430 |
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PLoS One |
Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study PubMed: 25955845 |
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Respir Care |
Diagnostic Utility of Biomarkers in COPD PubMed: 26106205 |
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Acta Anaesthesiologica Scandinavica |
Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472 |
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Journal of Clinical Medicine |
Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327 |
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Journal of Proteomics |
Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945 |
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Diagnostics |
Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848 |
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Pediatrics International |
Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus 34559910 |
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Catalog No. |
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Applications (RESEARCH USE ONLY!) |
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Enzyme-linked immunosorbent assay for Antigen Detection. |
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KSB697Hu01 |
Main materials for "Do It (ELISA Kit) Yourself". |
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