Cloud-clone
SKU:LAA123Hu71
Biotin-Linked Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)
Biotin-Linked Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA123Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against TGFa. It has been selected for its ability to recognize TGFa in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Journal of Physiology and Biochemistry |
Protective effects of curcumin, α-lipoic acid, and N-acetylcysteine against carbon tetrachloride-induced liver fibrosis in rats SpringerLink: h875w73171w2147w |
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Oncology Reports |
A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitro PubMed: PMC3583590 |
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Sci Total Environ. |
The effects of particulate matter on inflammation of respiratory system: Differences betweenmale and female. pubmed:28174048 |
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International Journal of Biological Sciences |
CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17 |
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JOURNAL OF CELLULAR AND MOLECULAR MEDICINE |
Polarization of ADAM17‐driven EGFR signalling in electric field‐guided collective migration of epidermal sheets Pubmed: 33164313 |
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Toll-like receptor 5-mediated signaling enhances liver regeneration in mice |
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Catalog No. |
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Main materials for "Do It (ELISA Kit) Yourself". |
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