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SKU:LAB864Hu71

Biotin-Linked Polyclonal Antibody to Lactate Dehydrogenase (LDH)

Biotin-Linked Polyclonal Antibody to Lactate Dehydrogenase (LDH)

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UOM
Concentration
Organism species

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Product No.

LAB864Hu71

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

n/a

Buffer Formulation

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LDH. It has been selected for its ability to recognize LDH in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Neuroscience Research

Hydrogen sulfide antagonizes homocysteine-induced neurotoxicity in PC12 cells PubMed: 20674619

Clinical and Experimental Pharmacology and Physiology

Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity PubMed: 21261675

Neurochemistry International

Hydrogen sulfide prevents formaldehyde-induced neurotoxicity to PC12 cells by attenuation of mitochondrial dysfunction and pro-apoptotic potential ScienceDirect: S0197018612001428

ostaglandins Leukot Essent Fatty Acids.

Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. Pubmed: 24095588

Journal of Diabetes and its Complications

Nerve growth factor rescues diabetic mice heart after ischemia/reperfusion injury via up-regulation of the TRPV1 receptor Pubmed:25650182

Cancer Science & Therapy

An Additional risk of Lung Cancer from Recurrent Exposure to Ethyl Carbamate (EC) in BALB/C Mice Open-Access: 1000375

Journal of Antimicrobial Chemotherapy

NLF20: an antimicrobial peptide with therapeutic potential against invasive Pseudomonas aeruginosa infection PubMed: 26503666

American Journal of Physology Heart and Circulatory Physiology

Dysfunction of mitochondria and deformed gap junctions in the heart of IL-18-deficient mice Pubmed:27288439

Translational Research

Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules Pubmed:27172385

Experimental Cell Research

UpreCavia (Guinea pig )lation of CBS/H2S system contributes to asymmetric dimethylarginine-triggered protection against the neurotoxicity of glutamate to PC12 cells by inhibiting NOS/NO pathway science:S0014482716301598

Blood Cells Mol Dis

Transgenic sickle cell trait mice do not exhibit abnormal thermoreCavia (Guinea pig )latory and stress responses to heat shock exposure Pubmed:27282581

PLoS One.

Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. pubmed:27907155

Scientific Reports

Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. pubmed:27678302

Experimental Biology and Medicine

Ameliorating effect of anti-Alzheimer’s drugs on the bidirectional association between type 2 diabetes mellitus and Alzheimer’s disease pubmed:28534431

Cancer Science

A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme … Scholarena:Source

Molecular Medicine Reports

Protective effects of N (2)‑L‑alanyl‑L‑glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion Pubmed:29393473

Molecular Medicine Reports 

Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats Pubmed:29956781

Oncology Letters

A novel potential effective strategy for enhancing the antitumor immune response in breast cancer patients using a viable cancer cell‑dendritic cell‑based vaccine Pubmed:29928442

Experimental Allergy and Immunology

Osteopontin protects against pneumococcal infection in a murine model of allergic airway inflammation Pubmed: 30362569

Scientific Reports

Low plasma haptoglobin is a risk factor for life-threatening childhood severe malarial anemia and not an exclusive consequence of hemolysis Pubmed: 30510258

Molecular Medicine Reports

Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury Pubmed: 32705277

Cell Transplant

Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stromal Cells Protect Cardiac Cells Against Hypoxia/Reoxygenation Injury by Inhibiting … Pubmed: 32864999

Nanomaterials

Biological Safety and Biodistribution of Chitosan Nanoparticles Pubmed: 32340313

Journal of Renal Injury Prevention

Prevention of kidney injury after myocardial ischemia reperfusion is achievable with short-term protein restriction

clinical and translational medicine

Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis 33634976

Biomed Res Int

Factors Affecting the Incidence, Progression, and Severity of COVID-19 in Type 1 Diabetes Mellitus 34840966

Cell Cycle

Improvement of autophagic flux mediates the protection of hydrogen sulfide against arecoline-elicited neurotoxicity in PC12 cells Pubmed:35316162

Biomed Res Int

A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating … Pubmed:35281603

Int Immunopharmacol

LncRNA KCNQ1OT1 attenuates myocardial injury induced by hip fracture via regulating of miR-224-3p/GATA4 axis Pubmed:35217336


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