Cloud-clone
SKU:LAA632Hu71
Biotin-Linked Polyclonal Antibody to Pepsin (PP)
Biotin-Linked Polyclonal Antibody to Pepsin (PP)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA632Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PP. It has been selected for its ability to recognize PP in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Radiology and Oncology |
LEVEL OF PEPSIN AND BILE ACIDS IN THE SALIVA OF PATIENTS WITH GLOTTIS T1 CARCINOMA Szd:Source |
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Radiology and Oncology |
Higher levels of total pepsin and bile acids in the saliva as a possible risk factor for early laryngeal cancer Pubmed:Pmc4362607 |
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Clin Otolaryngol |
Pepsin and bile acids in saliva in patients with laryngopharyngeal reflux – a prospective comparative study PubMed: 25516364 |
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journal of pediatric gastroenterology and nutrition |
Does Positioning Affect Tracheal Aspiration of Gastric Content in Ventilated Infants? PubMed: 25313850 |
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Journal of Voice |
Detecting Laryngopharyngeal Reflux by Immunohistochemistry of Pepsin in the Biopsies of Vocal Fold Leukoplakia pubmed:28756936 |
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Sensors (Basel) |
Label-Free Detection of Salivary Pepsin Using Gold Nanoparticle/Polypyrrole Nanocoral Modified Screen-Printed Electrode Pubmed:29882917 |
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The Tohoku Journal of Experimental Medicine |
Association between Pepsin in Bronchoalveolar Lavage Fluid and Prognosis of Chronic Fibrosing Interstitial Lung Disease |
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Respiratory Research |
Pulmonary aspiration in preschool children with cystic fibrosis Pubmed: 30558606 |
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Tanaffos |
Association of Bile Acid and Pepsin Micro-aspiration with Chronic Obstructive Pulmonary Disease Exacerbation Pubmed: 31423141 |
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Sensors |
Optimization of Saliva Collection and Immunochromatographic Detection of Salivary Pepsin for Point-of-Care Testing of Laryngopharyngeal Reflux Pubmed: 31935973 |
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BIOSCIENCE REPORTS |
Presence of pepsin in laryngeal tissue and saliva in benign and malignant neoplasms Pubmed: 33103719 |
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OTOLARYNGOLOGY-HEAD AND NECK SURGERY |
Relationship Between Pepsin Expression and Dysplasia Grade in Patients With Vocal Cord Leukoplakia Pubmed: 32692278 |
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American Journal of Otolaryngology |
Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps Pubmed: 32889371 |
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The relationship between laryngopharyngeal reflux based on pepsin value and clinical characteristics of laryngeal cancer patients |
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Med Sci Monit |
The Role of Salivary Pepsin in the Diagnosis of Gastroesophageal Reflux Disease (GERD) Evaluated Using High-Resolution Manometry and 24-Hour?¡ 33220027 |
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Annals of Esophagus |
Pepsin properties, structure, and its accurate measurement: a narrative review |
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Laryngoscope |
The Impact of Laryngopharyngeal Reflux on Occurrence and Clinical Course of Recurrent Respiratory Papillomatosis 34338331 |
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Pulmonology |
Bronchoalveolar lavage (BAL) amylase and pepsin levels as potential biomarkers of aspiration pneumonia Pubmed:35715334 |
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Neuromodulation: Technology at the Neural Interface |
Transcutaneous Electrical Acustimulation Improved the Quality of Life in Patients With Diarrhea-Irritable Bowel Syndrome Pubmed:35088760 |
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