Cloud-clone
SKU:LAB932Hu71
Biotin-Linked Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Biotin-Linked Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB932Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Clin Cancer Res |
Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402 |
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Clin Cancer Res |
Inactivation of the p53–KLF10–CEBPA Axis in Acute Myeloid Leukemia PubMed: 26408402 |
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Diabetes & Metabolism |
Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. pubmed:28499695 |
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Scientific Reports |
Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression pubmed:28720868 |
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Journal of Biological Chemistry |
Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids Pubmed: 30237167 |
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Biomaterials |
Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels Pubmed: 30343256 |
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Bulletin of Experimental Biology and Medicine |
Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells Pubmed: 30488202 |
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Journal of Medicinal Chemistry |
Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors Pubmed: 30938999 |
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International Immunopharmacology |
Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway Pubmed: 31078923 |
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Cancers |
Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia Pubmed: 31718075 |
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Biological research |
Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … Pubmed: 32046779 |
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Biomed Pharmacother |
Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway Pubmed: 32028241 |
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Metab Brain Dis |
Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats Pubmed: 33170419 |
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South African Journal of Botany |
Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo |
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Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice |
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Cancers (Basel) |
BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 33540760 |
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MOLECULAR CANCER RESEARCH |
Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity 33262291 |
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Sci Rep |
Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells 34837026 |
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Cancer Rep (Hoboken) |
Curcumin alters distinct molecular pathways in breast cancer subtypes revealed by integrated miRNA/mRNA expression analysis Pubmed:34981672 |
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Prog Neurobiol |
Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration Pubmed:35151792 |
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Plant Molecular Biology |
Arabidopsis GELP7 functions as a plasma membrane-localized acetyl xylan esterase, and its overexpression improves saccharification efficiency Pubmed:35577991 |
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