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SKU:LAA876Hu71

Biotin-Linked Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP)

Biotin-Linked Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP)

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UOM
Concentration
Organism species

Western blotting: 0.5-2µg/mL
;Immunohistochemistry: 5-20µg/mL
;Immunocytochemistry: 5-20µg/mL
;Optimal working dilutions must be determined by end user.

Product No.

LAA876Hu71

Organism Species

Homo sapiens (Human).

Source

Antibody labeling

Ig Type

IgG

Purification

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Label

Biotin

Original Antibody

PAA876Hu01-Polyclonal Antibody to Calcitonin Gene Related Peptide (CGRP)

Buffer Formulation

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Traits

Liquid

Concentration

500µg/mL

Organism Species More

n/a

Applications

WB; IHC; ICC.

If the antibody is used in flow cytometry, please check FCM antibodies.

UOM

20µl 100µl 200µl 1ml 10ml

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CGRP. It has been selected for its ability to recognize CGRP in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Magazine

Citations

Evidence-Based Complementary and Alternative Medicine

Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo. Hindawi: 190370

The Journal of Headache and Pain

Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological marker PubMed: PMC3620076

Headache: The Journal of Head and Face Pain

CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine Onlinelibrary: head.12372

PLoS One.

Ultraviolet B Irradiation Reduces the Expression of Adiponectin in Ovarial Adipose Tissues through Endocrine Actions of Calcitonin Gene-Related Peptide-Induced Serum Amyloid A Pubmed:Pmc4028234

Int J Vasc Med.

Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot. Pubmed:24624298

Arch Gynecol Obstet.

Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study Pubmed:24943060

Pain

OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine PubMed: 25735000

Molecular Medicine Reports

Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats PubMed: 25892548

Annals of Allergy, Asthma and Immunology

Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis Pubmed:27156748

Cephalalgia.

Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients Pubmed:27250233

Cardiology. 

Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction. Pubmed:27144592

J Headache Pain

Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB Pubmed:27150105

Frontiers in Physiology

Prevention of Bone Loss in a Model of Postmenopausal Osteoporosis throughAdrenomedullin Inhibition. pubmed:27445864

Naunyn Schmiedebergs Arch Pharmacol.

Opioid receptor agonists may favorably affect bone mechanical properties in rats with estrogen deficiency-induced osteoporosis. pubmed:27896372

American Journal of Translational Research

A new comorbidity model and the common pathological mechanisms of migraine and epilepsy pubmed:28559979

9

Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome pubmed:28483500

Journal of Clinical Investigation

CNS myeloid cells critically regulate heat hyperalgesia Pubmed:29634489

Journal of Headache and Pain

Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisal Pubmed:30006780

BioRxiv - the preprint server for biology

RAMP1 in Kupffer cells is a critical regulator in immune-mediated hepatitis 10.1101:357582

International Journal of Pharmaceutical Sciences and Research

MOTION SICKNESS INDUCES PHYSIOLOGICAL AND NEURONAL ALTERATIONS IN MOUSE MODEL

JOURNAL OF PERIODONTOLOGY

Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraine Pubmed: 31070784

Molecular and Cellular Biochemistry

Rhynchophylline attenuates migraine in trigeminal nucleus caudalis in nitroglycerin-induced rat model by inhibiting MAPK/NF-кB signaling Pubmed: 31420791

Journal of Headache and Pain

Electrical stimulation of the superior sagittal sinus suppresses A-type K+ currents and increases P/Q-and T-type Ca 2+ currents in rat trigeminal ganglion …

Respiratory Physiology & Neurobiology

Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma Pubmed: 31542455

Europe PMC

Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion … Pubmed: 31375062

Clinical Chemistry and Laboratory Medicine

Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headache Pubmed: 32286239

MOLECULAR BIOLOGY REPORTS

Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice Pubmed: 32951099

Scientific Reports

Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs 33441852

Nature Communications

Perirenal adipose afferent nerves sustain pathological high blood pressure in rats Pubmed:35668093


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