Cloud-clone
SKU:LAL917Hu71
Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A)
Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAL917Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against SEMA3A. It has been selected for its ability to recognize SEMA3A in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Arthritis Research & Therapy |
Semaphorin 3A is a marker for disease activity and a potential immunoregulator in systemic lupus erythematosus Biomed: ar3881 |
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journal of invastigative dermatology |
Cathelicidin LL-37 Induces Semaphorin 3A Expression in Human Epidermal Keratinocytes: Implications for Possible Application to Pruritus PubMed: 26121211 |
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Neurology |
Neurofilament light chain level is a weak risk factor for the development of MS. pubmed:27521440 |
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Development |
miR-126-5p promotes retinal endothelial cell survival through SetD5 regulatio in neurons pubmed:29180574 |
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A Preliminary Study of the Effect of Semaphorin 3A and Acitretin on the Proliferation, Migration, and Apoptosis of HaCaT Cells |
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Life Sciences |
Curcumin and LOXblock-1 ameliorate ischemia-reperfusion induced inflammation and acute kidney injury by suppressing the semaphorin-plexin pathway. Pubmed: 32603817 |
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ACS NANO |
Gold Nanoparticles Induce Tumor Vessel Normalization and Impair Metastasis by Inhibiting Endothelial Smad2/3 Signaling Pubmed: 32413258 |
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PLoS One |
Extract of Scutellaria baicalensis induces semaphorin 3A production in human epidermal keratinocytes 33905439 |
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Catalog No. |
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