Cloud-clone
SKU:LAA105Hu71
Biotin-Linked Polyclonal Antibody to Nerve Growth Factor (NGF)
Biotin-Linked Polyclonal Antibody to Nerve Growth Factor (NGF)
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Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAA105Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
PAA105Hu01-Polyclonal Antibody to Nerve Growth Factor (NGF)
Buffer Formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NGF. It has been selected for its ability to recognize NGF in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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The Tohoku Journal of Experimental Medicine |
Low-intensity aerobic exercise mitigates exercise-induced bronchoconstriction by improving the function of adrenal medullary chromaffin cells in asthmatic rats Pubmed:25212248 |
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International Journal of Pharmaceutics |
Neurotrophic and antioxidant effects of silymarin comparable to 4-methylcatechol in protection against gentamicin-induced ototoxicity in guinea pigs Pubmed:25445537 |
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Yonsei Medical Journal |
NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome Pubmed:25510766 |
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Molecular and Cellular Endocrinology |
High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 Pubmed:25614971 |
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Mol Cell Endocrinol |
High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 PubMed: 25614971 |
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Neurosciences (Riyadh) |
Telmisartan inhibits hyperalgesia and inflammatory progression in a diabetic neuropathic pain model of Wistar rats. PubMed: 25864063 |
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Neurotoxicity Research |
Pro-apoptotic Action of Corticosterone in Hippocampal Organotypic Cultures Pubmed:27189478 |
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Psychoneuroendocrinology |
Chronic mild stress influences nerve growth factor through a matrix metalloproteinase-dependent mechanism Pubmed:26771945 |
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Genet Mol Res |
Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells Pubmed:27323064 |
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The Journal of Maternal-Fetal & Neonatal Medicine |
Differential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalities pubmed:28081639 |
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IOS Press Content Library |
An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … articles:journal-of-alzheimers-disease |
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biblioteca.uva |
Evaluación de la secreción de factores neurotróficos por las células MSV en cocultivo con neurorretinas porcinas, en función de la dosis celular y del tiempo de cultivo 10324/27440 |
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Progress in Neuro-Psychopharmacology & Biological Psychiatry |
Antidepressant drugs for beta amyloid-induced depression: A new standpoint? pubmed:28499898 |
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Osteoarthritis and Cartilage |
Mechanical loading of intervertebral disc modulates microglia proliferation, activation, and chemotaxis Pubmed:29723636 |
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Stem Cell Research & Therapy |
Autophagy is required for human umbilical cord mesenchymal stem cells to improve spatial working memory in APP/PS1 transgenic mouse model Pubmed:29335016 |
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Pharmacological Research |
N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype Pubmed:29203442 |
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Experimental Neurology |
Preterm umbilical cord blood derived mesenchymal stem/stromal cells protect preterm white matter brain development against hypoxia-ischemia Pubmed:30012511 |
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Biochemical Pharmacology |
Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in naïve and Aβ-treated female rats Pubmed:30028991 |
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Experimental Dermatology |
3‐‐Laurylglyceryl ascorbate improves the development of sensitive skin through the reduction of oxidative stress Pubmed: 30698879 |
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BioMed Research International |
Administration of Repetitive Transcranial Magnetic Stimulation Attenuates Aβ1-42-Induced Alzheimer's Disease in Mice by Activating β-Catenin Signaling |
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International Journal of Ophthalmology |
A novel xeno-free culture system for human retinal pigment epithelium cells Pubmed: 31024807 |
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Experimental and Therapeutic Medicine |
Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity Pubmed: 32010279 |
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Open Access Macedonian Journal of Medical Sciences |
Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy Pubmed: 32165929 |
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MOLECULAR NUTRITION & FOOD RESEARCH |
Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and … Pubmed: 32918393 |
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Neuropharmacology |
Impaired LTD-like motor cortical plasticity in female patients with major depression disorder Pubmed: 32791084 |
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COLLOIDS AND SURFACES B-BIOINTERFACES |
Effects of gold nanoparticles administration through behavioral and oxidative parameters in animal model of parkinson's disease Pubmed: 32777662 |
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博士论文 |
Neurobiological determinants of depressive-like symptoms in rodents |
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Medical Research Journal |
Changes of substance P, NGF and CGRP salivary levels among patients undergoing physical therapy |
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Journal of the Taiwan Institute of Chemical Engineers |
Multiple-component dual-phase solid lipid nanoparticles with conjugated transferrin for formulating antioxidants and nerve growth factor against neuronal apoptosis |
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EUROPEAN CHILD & ADOLESCENT PSYCHIATRY |
Increased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡ 33389158 |
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DRUG DEVELOPMENT RESEARCH |
Neuroprotective effect of Na+/H+?exchangers isoform©\1 inactivation against 6©\hydroxydopamine©\induced mitochondrial dysfunction and neuronal apoptosis in Parkinson's disease models 33538000 |
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BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY |
Sea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways 33589896 |
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Neuropsychiatr Dis Treat |
Repetitive Transcranial Magnetic Stimulation Improves Mild Cognitive Impairment Associated with Alzheimer's Disease in Mice by Modulating the miR-567 … 34239303 |
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Pharmaceuticals (Basel) |
Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers 34959705 |
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Cell Biochem Funct |
CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway 34296452 |
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Carbohydrate Polymers |
Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration Pubmed:35550777 |
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Catalog No. |
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