Cloud-clone
SKU:LAB040Hu71
Biotin-Linked Polyclonal Antibody to Vimentin (VIM)
Biotin-Linked Polyclonal Antibody to Vimentin (VIM)
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Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAB040Hu71
Organism Species
Homo sapiens (Human).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
Biotin
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against VIM. It has been selected for its ability to recognize VIM in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
| Magazine | Citations |
| Cancer Epidemiology, Biomarkers & Prevention | Aberrant Vimentin Methylation Is Characteristic of Breast Cancer pubmed:22315367 |
| Scientific Reports | Glyoxalase 1-knockdown in human aortic endothelial cells - effect on the proteome andendothelial function estimates. pubmed:27898103 |
| Journal of Proteomics | HtrA3 is a cellular partner of cytoskeleton proteins and TCP1α chaperonin Pubmed:29477555 |
| Journal of Biological Chemistry | Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328 |
| American Journal of Transplantation | Extracellular vesicles derived from injured vascular tissue promote the formation of tertiary lymphoid structures in vascular allografts Pubmed: 31729155 |
| Lung | Is Vimentin the Cause or Effect of Obstructive Sleep Apnea Development? Pubmed: 32088750 |
| Cell Biol Toxicol | ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer 33566221 |
| CELL BIOLOGY AND TOXICOLOGY | Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence 33758996 |
| Comparison of Vimentin Levels Between Preeclamptic and Normotensive Pregnant Women | |
| Advanced glycation end-products associate with podocytopathy in type II diabetic patients | |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPB040Hu02 | Recombinant Vimentin (VIM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB040Hu01 | Active Vimentin (VIM) | Cell culture; Activity Assays. |
| RPB040Hu01 | Recombinant Vimentin (VIM) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB040Hu02 | Polyclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| PAB040Hu01 | Polyclonal Antibody to Vimentin (VIM) | WB; IHC; IF |
| LAB040Hu71 | Biotin-Linked Polyclonal Antibody to Vimentin (VIM) | WB; IHC; ICC. |
| MAB040Hu24 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC |
| MAB040Hu25 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; IF |
| MAB040Hu21 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| MAB040Hu23 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| MAB040Hu27 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; ICC; IP. |
| MAB040Hu22 | Monoclonal Antibody to Vimentin (VIM) | WB; IHC; IF |
| SEB040Hu | ELISA Kit for Vimentin (VIM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB040Hu | Magnetic Luminex Assay Kit for Vimentin (VIM) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
| KSB040Hu01 | ELISA Kit DIY Materials for Vimentin (VIM) | Main materials for "Do It (ELISA Kit) Yourself" |
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